[article] 
inResearch in Autism Spectrum Disorders > 5-1 (January-March 2011) . - p.286-293

Titre :	Metals, metallothioneins and oxidative stress in blood of autistic children
Type de document :	Texte imprimé et/ou numérique
Auteurs :	Laura VERGANI, Auteur ; Laura CANESI, Auteur ; Elena GRASSELLI, Auteur ; Giuseppe MINNITI, Auteur ; Edvige VENESELLI, Auteur ; Genti SHYTI, Auteur ; Luisa M. ABELMOSCHI, Auteur ; Paola RIVARO, Auteur ; Cristina LANZA, Auteur ; Adriana VOCI, Auteur
Année de publication :	2011
Article en page(s) :	p.286-293
Langues :	Anglais (eng)
Mots-clés :	Autistic-spectrum-disorders Blood Metals Metallothionein Oxidative-stress Real-time-RT-PCR
Index. décimale :	PER Périodiques
Résumé :	Many factors have been implicated in autism onset, including excess or deficiency in toxic or essential metals and impaired antioxidant systems. Protection towards the damaging effects of reactive oxygen species (ROS) is afforded by antioxidant enzymes (superoxide dismutase, SOD, catalase, CAT, glutathione peroxidase, GPx), and non-enzymatic antioxidants such as metallothionein (MT). In blood samples from autistic children and healthy controls, three aspects were investigated: (i) the plasma concentration of 13 metals (Al, As, Ca, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Si, Zn) was measured by ICP-AES; (ii) the transcription of three MT isoforms (MT-1A, MT-1E and MT-2A) was assessed by real-time RT-PCR in peripheral blood leucocytes (PBMCs); (iii) the activity of SOD, CAT and GPx was assessed by spectrophotometric assays in red blood cells (RBCs). Autistic children showed significantly higher plasma levels of Zn, Ca, Fe, As, Ni, Cd and Si, higher mRNA expression of the MT isoforms in PBMCs, higher SOD activity and lower CAT activity in RBCs with respect to controls. These findings demonstrate a significant increase in both metal content and metallothionein expression together with an unbalance in the oxidative status in the blood of autistic children.
En ligne :	http://dx.doi.org/10.1016/j.rasd.2010.04.010
Permalink :	https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=111

[article] Metals, metallothioneins and oxidative stress in blood of autistic children [Texte imprimé et/ou numérique] / Laura VERGANI, Auteur ; Laura CANESI, Auteur ; Elena GRASSELLI, Auteur ; Giuseppe MINNITI, Auteur ; Edvige VENESELLI, Auteur ; Genti SHYTI, Auteur ; Luisa M. ABELMOSCHI, Auteur ; Paola RIVARO, Auteur ; Cristina LANZA, Auteur ; Adriana VOCI, Auteur . - 2011 . - p.286-293.
Langues : Anglais (eng)
in Research in Autism Spectrum Disorders > 5-1 (January-March 2011) . - p.286-293

Mots-clés :	Autistic-spectrum-disorders Blood Metals Metallothionein Oxidative-stress Real-time-RT-PCR
Index. décimale :	PER Périodiques
Résumé :	Many factors have been implicated in autism onset, including excess or deficiency in toxic or essential metals and impaired antioxidant systems. Protection towards the damaging effects of reactive oxygen species (ROS) is afforded by antioxidant enzymes (superoxide dismutase, SOD, catalase, CAT, glutathione peroxidase, GPx), and non-enzymatic antioxidants such as metallothionein (MT). In blood samples from autistic children and healthy controls, three aspects were investigated: (i) the plasma concentration of 13 metals (Al, As, Ca, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Si, Zn) was measured by ICP-AES; (ii) the transcription of three MT isoforms (MT-1A, MT-1E and MT-2A) was assessed by real-time RT-PCR in peripheral blood leucocytes (PBMCs); (iii) the activity of SOD, CAT and GPx was assessed by spectrophotometric assays in red blood cells (RBCs). Autistic children showed significantly higher plasma levels of Zn, Ca, Fe, As, Ni, Cd and Si, higher mRNA expression of the MT isoforms in PBMCs, higher SOD activity and lower CAT activity in RBCs with respect to controls. These findings demonstrate a significant increase in both metal content and metallothionein expression together with an unbalance in the oxidative status in the blood of autistic children.
En ligne :	http://dx.doi.org/10.1016/j.rasd.2010.04.010
Permalink :	https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=111