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Auteur Brian M. CAMPBELL |
Documents disponibles écrits par cet auteur (3)
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Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis / Diane T. STEPHENSON in Molecular Autism, (May 2011)
[article]
Titre : Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis Type de document : Texte imprimé et/ou numérique Auteurs : Diane T. STEPHENSON, Auteur ; Sharon M. O'NEILL, Auteur ; Sapna NARAYAN, Auteur ; Aadhya TIWARI, Auteur ; Elizabeth ARNOLD, Auteur ; Harry SAMAROO, Auteur ; Fu DU, Auteur ; Robert RING, Auteur ; Brian M. CAMPBELL, Auteur ; Mathew PLETCHER, Auteur ; Vidita A. VAIDYA, Auteur ; Daniel MORTON, Auteur Année de publication : 2011 Article en page(s) : 76 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Background
The inbred mouse strain, BTBR T+ tf/J (BTBR) exhibits behavioral deficits which mimic the core deficits of autism. Neuroanatomically, the BTBR strain is also characterized by a complete absence of the corpus callosum. The goal of this study was to identify novel molecular and cellular changes in the BTBR mouse focusing on, neuronal, synaptic, glial and plasticity markers in the limbic system as a model for identifying putative molecular and cellular substrates associated with autistic behaviors. .
Methods
Forebrains of 8 to 10 week old male BTBR and aged matched C57Bl/6J controls were evaluated by immunohistochemistry using free floating and paraffin embedded sections. Twenty antibodies directed to antigens specific to neurons, synapses and glia were used. Nissl, Timm's and acetylcholinesterase (AchE) stains were performed to assess cytoarchitecture, mossy fibers and cholinergic fiber density, respectively. In the hippocampus, quantitative stereological estimates for the mitotic marker bromodeoxyuridine were performed to determine hippocampal progenitor proliferation, survival and differentiation and BDNF mRNA was quantified by in situ hybridization. Quantitative image analysis was performed for NG2, doublecortin, NeuroD, GAD67 and PSA-NCAM.
Results
In midline structures including the region of the absent corpus callosum of BTBR mice, myelin markers myelin basic protein (MBP) and 2' , 3' -cyclic nucleotide 3' -phosphodiesterase (CNPase) were reduced and oligodendrocyte precursor NG2 was increased. MBP and CNPase were expressed in small ectopic white matter bundles within the cingulate cortex. Microglia and astrocytes showed no evidence of gliosis, yet orientations of glial fibers were altered in specific white matter areas. In the hippocampus, evidence of reduced neurogenesis included significant reductions in the number of doublecortin, PSA-NCAM, and NeuroD immunoreactive cells in the subgranular zone of the dentate gyrus and a marked reduction in the number of BrdU positive progenitors Furthermore, a significant and profound reduction in BDNF mRNA in the BTBR dentate gyrus was observed. No significant differences were observed in the expression of acetylcholinesterase, mossy fiber synapses, and immunoreactivities for MAP2, parvalbumin, GAD65 and GAD67.
Conclusions
We documented modest and selective alterations in glia, neurons and synapses in BTBR forebrain, along with reduced adult hippocampal neurogenesis. Of all markers examined, the most distinctive changes were observed in the neurodevelopmental proteins NG2, PSA-NCAM, NeuroD and doublecortin. Our results are consistent with aberrant development of the nervous system in BTBR mice and may reveal novel substrates to link callosal abnormalities and autistic behaviors. The changes that we observed in the BTBR mice suggest potential novel therapeutic strategies for intervention in autism spectrum disorders.En ligne : http://dx.doi.org/10.1186/2040-2392-2-7 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131
in Molecular Autism > (May 2011) . - 76 p.[article] Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis [Texte imprimé et/ou numérique] / Diane T. STEPHENSON, Auteur ; Sharon M. O'NEILL, Auteur ; Sapna NARAYAN, Auteur ; Aadhya TIWARI, Auteur ; Elizabeth ARNOLD, Auteur ; Harry SAMAROO, Auteur ; Fu DU, Auteur ; Robert RING, Auteur ; Brian M. CAMPBELL, Auteur ; Mathew PLETCHER, Auteur ; Vidita A. VAIDYA, Auteur ; Daniel MORTON, Auteur . - 2011 . - 76 p.
Langues : Anglais (eng)
in Molecular Autism > (May 2011) . - 76 p.
Index. décimale : PER Périodiques Résumé : Background
The inbred mouse strain, BTBR T+ tf/J (BTBR) exhibits behavioral deficits which mimic the core deficits of autism. Neuroanatomically, the BTBR strain is also characterized by a complete absence of the corpus callosum. The goal of this study was to identify novel molecular and cellular changes in the BTBR mouse focusing on, neuronal, synaptic, glial and plasticity markers in the limbic system as a model for identifying putative molecular and cellular substrates associated with autistic behaviors. .
Methods
Forebrains of 8 to 10 week old male BTBR and aged matched C57Bl/6J controls were evaluated by immunohistochemistry using free floating and paraffin embedded sections. Twenty antibodies directed to antigens specific to neurons, synapses and glia were used. Nissl, Timm's and acetylcholinesterase (AchE) stains were performed to assess cytoarchitecture, mossy fibers and cholinergic fiber density, respectively. In the hippocampus, quantitative stereological estimates for the mitotic marker bromodeoxyuridine were performed to determine hippocampal progenitor proliferation, survival and differentiation and BDNF mRNA was quantified by in situ hybridization. Quantitative image analysis was performed for NG2, doublecortin, NeuroD, GAD67 and PSA-NCAM.
Results
In midline structures including the region of the absent corpus callosum of BTBR mice, myelin markers myelin basic protein (MBP) and 2' , 3' -cyclic nucleotide 3' -phosphodiesterase (CNPase) were reduced and oligodendrocyte precursor NG2 was increased. MBP and CNPase were expressed in small ectopic white matter bundles within the cingulate cortex. Microglia and astrocytes showed no evidence of gliosis, yet orientations of glial fibers were altered in specific white matter areas. In the hippocampus, evidence of reduced neurogenesis included significant reductions in the number of doublecortin, PSA-NCAM, and NeuroD immunoreactive cells in the subgranular zone of the dentate gyrus and a marked reduction in the number of BrdU positive progenitors Furthermore, a significant and profound reduction in BDNF mRNA in the BTBR dentate gyrus was observed. No significant differences were observed in the expression of acetylcholinesterase, mossy fiber synapses, and immunoreactivities for MAP2, parvalbumin, GAD65 and GAD67.
Conclusions
We documented modest and selective alterations in glia, neurons and synapses in BTBR forebrain, along with reduced adult hippocampal neurogenesis. Of all markers examined, the most distinctive changes were observed in the neurodevelopmental proteins NG2, PSA-NCAM, NeuroD and doublecortin. Our results are consistent with aberrant development of the nervous system in BTBR mice and may reveal novel substrates to link callosal abnormalities and autistic behaviors. The changes that we observed in the BTBR mice suggest potential novel therapeutic strategies for intervention in autism spectrum disorders.En ligne : http://dx.doi.org/10.1186/2040-2392-2-7 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131 Possible Evidence for a Fall in the Prevalence of High-Functioning Pervasive Developmental Disorder with Age? / M. BALFE in Autism Research and Treatment, (March 2011)
[article]
Titre : Possible Evidence for a Fall in the Prevalence of High-Functioning Pervasive Developmental Disorder with Age? Type de document : Texte imprimé et/ou numérique Auteurs : M. BALFE, Auteur ; Digby TANTAM, Auteur ; Brian M. CAMPBELL, Auteur Année de publication : 2011 Article en page(s) : 8 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : A survey was undertaken to investigate the prevalence of high-functioning pervasive developmental disorder (HFPDD) in a community sample of teenagers and adults aged 13 and above in the city of Sheffield, UK. 112 possible and definite cases were found, of whom 65 (57%) had a previous diagnosis. The detected prevalence of possible or definite HFPDD was found to be 0.24 per 1000 of the population of Sheffield city aged 13 or over, but the prevalence by year of age fell from a maximum of 1.1 per 1000 in the group aged 13 to 14 years old (1 young adult in every 900 in this age group) to 0.03 per 1000 in the over 60s (1 person in every 38500 in this age group). The results of this study are preliminary and need follow-up investigation in larger studies. We suggest several explanations for the findings, including reduced willingness to participate in a study as people get older, increased ascertainment in younger people, and increased mortality. Another contributory factor might be that the prevalence of high-functioning pervasive development disorder may decline with age. This raises the possibility that AS symptoms might become subclinical in adulthood in a proportion of people with HFPDD. En ligne : http://dx.doi.org/10.1155/2011/325495 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131
in Autism Research and Treatment > (March 2011) . - 8 p.[article] Possible Evidence for a Fall in the Prevalence of High-Functioning Pervasive Developmental Disorder with Age? [Texte imprimé et/ou numérique] / M. BALFE, Auteur ; Digby TANTAM, Auteur ; Brian M. CAMPBELL, Auteur . - 2011 . - 8 p.
Langues : Anglais (eng)
in Autism Research and Treatment > (March 2011) . - 8 p.
Index. décimale : PER Périodiques Résumé : A survey was undertaken to investigate the prevalence of high-functioning pervasive developmental disorder (HFPDD) in a community sample of teenagers and adults aged 13 and above in the city of Sheffield, UK. 112 possible and definite cases were found, of whom 65 (57%) had a previous diagnosis. The detected prevalence of possible or definite HFPDD was found to be 0.24 per 1000 of the population of Sheffield city aged 13 or over, but the prevalence by year of age fell from a maximum of 1.1 per 1000 in the group aged 13 to 14 years old (1 young adult in every 900 in this age group) to 0.03 per 1000 in the over 60s (1 person in every 38500 in this age group). The results of this study are preliminary and need follow-up investigation in larger studies. We suggest several explanations for the findings, including reduced willingness to participate in a study as people get older, increased ascertainment in younger people, and increased mortality. Another contributory factor might be that the prevalence of high-functioning pervasive development disorder may decline with age. This raises the possibility that AS symptoms might become subclinical in adulthood in a proportion of people with HFPDD. En ligne : http://dx.doi.org/10.1155/2011/325495 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131 A study of six cases of de Lange Amsterdam dwarf syndrome, with special attention to voice, speech and language characteristics / William I. FRASER in Developmental Medicine & Child Neurology, 20-2 (April 1978)
[article]
Titre : A study of six cases of de Lange Amsterdam dwarf syndrome, with special attention to voice, speech and language characteristics Type de document : Texte imprimé et/ou numérique Auteurs : William I. FRASER, Auteur ; Brian M. CAMPBELL, Auteur Année de publication : 1978 Article en page(s) : p.189-198 Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : From a survey of southern Scotland, six severly handicapped subjects (age-range 8 to 22 years) were established by firm criteria as suffering from the de Lange Amsterdam Dwarf syndrome. They showed a high incidence of behaviour disturbance. Language development was retarded and all but one of the subjects were dysphonic. A connection may exist between the glottal "fry" (an unperiodical phonation of the vocal folds in a frequency below the normal pitch register) observed in the cries of the younger non-speaking cases and the hoarseness in the speech of the older subjects. Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=476
in Developmental Medicine & Child Neurology > 20-2 (April 1978) . - p.189-198[article] A study of six cases of de Lange Amsterdam dwarf syndrome, with special attention to voice, speech and language characteristics [Texte imprimé et/ou numérique] / William I. FRASER, Auteur ; Brian M. CAMPBELL, Auteur . - 1978 . - p.189-198.
Langues : Anglais (eng)
in Developmental Medicine & Child Neurology > 20-2 (April 1978) . - p.189-198
Index. décimale : PER Périodiques Résumé : From a survey of southern Scotland, six severly handicapped subjects (age-range 8 to 22 years) were established by firm criteria as suffering from the de Lange Amsterdam Dwarf syndrome. They showed a high incidence of behaviour disturbance. Language development was retarded and all but one of the subjects were dysphonic. A connection may exist between the glottal "fry" (an unperiodical phonation of the vocal folds in a frequency below the normal pitch register) observed in the cries of the younger non-speaking cases and the hoarseness in the speech of the older subjects. Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=476