Gene expression analysis in lymphoblasts derived from patients with autism spectrum disorder / Yuka YASUDA in Molecular Autism, (May 2011)  

Public ISBD [article]  inMolecular Autism > (May 2011) . - 8 p. Titre : Gene expression analysis in lymphoblasts derived from patients with autism spectrum disorder Type de document : Texte imprimé et/ou numérique Auteurs : Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidenaga YAMAMORI, Auteur ; Kazutaka OHI, Auteur ; Motoyuki FUKUMOTO, Auteur ; Satomi UMEDA-YANO, Auteur ; Ikuko MOHRI, Auteur ; Akira ITO, Auteur ; Masako TANIIKE, Auteur ; Masatoshi TAKEDA, Auteur Année de publication : 2011 Article en page(s) : 8 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Background The autism spectrum disorders (ASDs) are complex neurodevelopmental disorders that result in severe and pervasive impairment in the development of reciprocal social interaction and verbal and nonverbal communication skills. In addition, individuals with ASD have stereotypical behavior, interests and activities. Rare mutations of some genes, such as neuroligin (NLGN) 3/4, neurexin (NRXN) 1, SHANK3, MeCP2 and NHE9, have been reported to be associated with ASD. In the present study, we investigated whether alterations in mRNA expression levels of these genes could be found in lymphoblastoid cell lines derived from patients with ASD. Methods We measured mRNA expression levels of NLGN3/4, NRXN1, SHANK3, MeCP2, NHE9 and AKT1 in lymphoblastoid cells from 35 patients with ASD and 35 healthy controls, as well as from 45 patients with schizophrenia and 45 healthy controls, using real-time quantitative reverse transcriptase polymerase chain reaction assays. Results The mRNA expression levels of NLGN3 and SHANK3 normalized by β-actin or TBP were significantly decreased in the individuals with ASD compared to controls, whereas no difference was found in the mRNA expression level of MeCP2, NHE9 or AKT1. However, normalized NLGN3 and SHANK3 gene expression levels were not altered in patients with schizophrenia, and expression levels of NLGN4 and NRXN1 mRNA were not quantitatively measurable in lymphoblastoid cells. Conclusions Our results provide evidence that the NLGN3 and SHANK3 genes may be differentially expressed in lymphoblastoid cell lines from individuals with ASD compared to those from controls. These findings suggest the possibility that decreased mRNA expression levels of these genes might be involved in the pathophysiology of ASD in a substantial population of ASD patients. En ligne : http://dx.doi.org/10.1186/2040-2392-2-9 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131 [article] Gene expression analysis in lymphoblasts derived from patients with autism spectrum disorder [Texte imprimé et/ou numérique] / Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidenaga YAMAMORI, Auteur ; Kazutaka OHI, Auteur ; Motoyuki FUKUMOTO, Auteur ; Satomi UMEDA-YANO, Auteur ; Ikuko MOHRI, Auteur ; Akira ITO, Auteur ; Masako TANIIKE, Auteur ; Masatoshi TAKEDA, Auteur . - 2011 . - 8 p. Langues : Anglais (eng) in Molecular Autism > (May 2011) . - 8 p. Index. décimale : PER Périodiques Résumé : Background The autism spectrum disorders (ASDs) are complex neurodevelopmental disorders that result in severe and pervasive impairment in the development of reciprocal social interaction and verbal and nonverbal communication skills. In addition, individuals with ASD have stereotypical behavior, interests and activities. Rare mutations of some genes, such as neuroligin (NLGN) 3/4, neurexin (NRXN) 1, SHANK3, MeCP2 and NHE9, have been reported to be associated with ASD. In the present study, we investigated whether alterations in mRNA expression levels of these genes could be found in lymphoblastoid cell lines derived from patients with ASD. Methods We measured mRNA expression levels of NLGN3/4, NRXN1, SHANK3, MeCP2, NHE9 and AKT1 in lymphoblastoid cells from 35 patients with ASD and 35 healthy controls, as well as from 45 patients with schizophrenia and 45 healthy controls, using real-time quantitative reverse transcriptase polymerase chain reaction assays. Results The mRNA expression levels of NLGN3 and SHANK3 normalized by β-actin or TBP were significantly decreased in the individuals with ASD compared to controls, whereas no difference was found in the mRNA expression level of MeCP2, NHE9 or AKT1. However, normalized NLGN3 and SHANK3 gene expression levels were not altered in patients with schizophrenia, and expression levels of NLGN4 and NRXN1 mRNA were not quantitatively measurable in lymphoblastoid cells. Conclusions Our results provide evidence that the NLGN3 and SHANK3 genes may be differentially expressed in lymphoblastoid cell lines from individuals with ASD compared to those from controls. These findings suggest the possibility that decreased mRNA expression levels of these genes might be involved in the pathophysiology of ASD in a substantial population of ASD patients. En ligne : http://dx.doi.org/10.1186/2040-2392-2-9 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=131

The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder / Sachie KANEKO in Autism Research, 13-6 (June 2020)  

Public ISBD [article]  inAutism Research > 13-6 (June 2020) . - p.947-958 Titre : The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder Type de document : Texte imprimé et/ou numérique Auteurs : Sachie KANEKO, Auteur ; Takahiro A. KATO, Auteur ; Manabu MAKINODAN, Auteur ; Takashi KOMORI, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Masato TAKAHASHI, Auteur ; Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidemi IWASAKA, Auteur ; Ayumi TANAKA, Auteur ; Yukiko UCHIDA, Auteur ; Shigenobu KANBA, Auteur ; Toshifumi KISHIMOTO, Auteur Article en page(s) : p.947-958 Langues : Anglais (eng) Mots-clés : attention deficit/hyperactivity disorder  autism spectrum disorder  heterogeneity  self-construal scale  well-being Index. décimale : PER Périodiques Résumé : Despite accumulating evidence that culture shapes the symptoms of autism spectrum disorder (ASD), no studies have yet applied the Self-Construal Scale to individuals with ASD. We compared the self-construals (measured using the Self-Construal Scale) of 31 high-functioning Japanese individuals with ASD with those of 60 typically developing (TD) individuals. We also examined how the self-construals of individuals with ASD related to their intelligence quotient, adverse childhood experiences, attention deficit hyperactivity disorder, ASD symptoms during adulthood and preschool years, and subjective well-being. Individuals with ASD were more likely to display independent self-construals than were TD individuals; unexpectedly, however, a substantial proportion of individuals with ASD (43.8%) displayed relatively interdependent self-construals. Among individuals with ASD, self-construals were significantly associated with ASD symptoms during preschool years, and with satisfaction of the need for autonomy and frustration of the need for relatedness. Evaluating self-construals can help predict the subjective well-being of high-functioning individuals with ASD. Moreover, the Self-Construal Scale may be useful for understanding the heterogeneous phenotypes of ASD, based on its association with autistic symptoms during preschool years, suggesting that the scale is a potential tool to develop efficient interventions for high-functioning individuals with ASD. Autism Res 2020, 13: 947-958. © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Autism Spectrum Disorders (ASD) are a group of disorders presenting a variety of symptoms and biological origins that can complicate choosing an intervention best suited for improving well-being. Results indicate that a self-construal scale could help understand individuals with high-functioning ASD by independent and interdependent self-construals that are associated with ASD symptoms during preschool years and adult subjective well-being. Our findings suggest that this scale can help understand ASD and select appropriate interventions. En ligne : http://dx.doi.org/10.1002/aur.2242 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=427 [article] The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder [Texte imprimé et/ou numérique] / Sachie KANEKO, Auteur ; Takahiro A. KATO, Auteur ; Manabu MAKINODAN, Auteur ; Takashi KOMORI, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Masato TAKAHASHI, Auteur ; Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidemi IWASAKA, Auteur ; Ayumi TANAKA, Auteur ; Yukiko UCHIDA, Auteur ; Shigenobu KANBA, Auteur ; Toshifumi KISHIMOTO, Auteur . - p.947-958. Langues : Anglais (eng) in Autism Research > 13-6 (June 2020) . - p.947-958 Mots-clés : attention deficit/hyperactivity disorder  autism spectrum disorder  heterogeneity  self-construal scale  well-being Index. décimale : PER Périodiques Résumé : Despite accumulating evidence that culture shapes the symptoms of autism spectrum disorder (ASD), no studies have yet applied the Self-Construal Scale to individuals with ASD. We compared the self-construals (measured using the Self-Construal Scale) of 31 high-functioning Japanese individuals with ASD with those of 60 typically developing (TD) individuals. We also examined how the self-construals of individuals with ASD related to their intelligence quotient, adverse childhood experiences, attention deficit hyperactivity disorder, ASD symptoms during adulthood and preschool years, and subjective well-being. Individuals with ASD were more likely to display independent self-construals than were TD individuals; unexpectedly, however, a substantial proportion of individuals with ASD (43.8%) displayed relatively interdependent self-construals. Among individuals with ASD, self-construals were significantly associated with ASD symptoms during preschool years, and with satisfaction of the need for autonomy and frustration of the need for relatedness. Evaluating self-construals can help predict the subjective well-being of high-functioning individuals with ASD. Moreover, the Self-Construal Scale may be useful for understanding the heterogeneous phenotypes of ASD, based on its association with autistic symptoms during preschool years, suggesting that the scale is a potential tool to develop efficient interventions for high-functioning individuals with ASD. Autism Res 2020, 13: 947-958. © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Autism Spectrum Disorders (ASD) are a group of disorders presenting a variety of symptoms and biological origins that can complicate choosing an intervention best suited for improving well-being. Results indicate that a self-construal scale could help understand individuals with high-functioning ASD by independent and interdependent self-construals that are associated with ASD symptoms during preschool years and adult subjective well-being. Our findings suggest that this scale can help understand ASD and select appropriate interventions. En ligne : http://dx.doi.org/10.1002/aur.2242 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=427

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