[article]
| Titre : |
Effects of a postnatal Atrx conditional knockout in neurons on autism-like behaviours in male and female mice |
| Type de document : |
texte imprimé |
| Auteurs : |
Nicole MARTIN-KENNY, Auteur ; Nathalie G. BÉRUBÉ, Auteur |
| Langues : |
Anglais (eng) |
| Mots-clés : |
Animals Autistic Disorder/genetics Chromatin Assembly and Disassembly Female Male X-Linked Intellectual Disability/genetics Mice Mice, Knockout Mutation Neurons/metabolism Postpartum Period X-linked Nuclear Protein alpha-Thalassemia/genetics Atrx Autism spectrum disorder Cre/loxP system Genetically engineered mice Repetitive behaviours Social behaviours Startle response |
| Index. décimale : |
PER Périodiques |
| Résumé : |
BACKGROUND: Alpha-thalassemia/mental retardation, X-linked, or ATRX, is an autism susceptibility gene that encodes a chromatin remodeler. Mutations of ATRX result in the ATR-X intellectual disability syndrome and have been identified in autism spectrum disorder (ASD) patients. The mechanisms by which ATRX mutations lead to autism and autistic-like behaviours are not yet known. To address this question, we generated mice with postnatal Atrx inactivation in excitatory neurons of the forebrain and performed a battery of behavioural assays that assess autistic-like behaviours. METHODS: Male and female mice with a postnatal conditional ablation of ATRX were generated using the Cre/lox system under the control of the αCaMKII gene promoter. These mice were tested in a battery of behavioural tests that assess autistic-like features. We utilized paradigms that measure social behaviour, repetitive, and stereotyped behaviours, as well as sensory gating. Statistics were calculated by two-way repeated measures ANOVA with Sidak's multiple comparison test or unpaired Student's t tests as indicated. RESULTS: The behaviour tests revealed no significant differences between Atrx-cKO and control mice. We identified sexually dimorphic changes in odor habituation and discrimination; however, these changes did not correlate with social deficits. CONCLUSION: The postnatal knockout of Atrx in forebrain excitatory neurons does not lead to autism-related behaviours in male or female mice. |
| En ligne : |
https://dx.doi.org/10.1186/s11689-020-09319-0 |
| Permalink : |
https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=573 |
in Journal of Neurodevelopmental Disorders > 12 (2020)
|  |
Effects of a postnatal Atrx conditional knockout in neurons on autism-like behaviours in male and female mice in Journal of Neurodevelopmental Disorders, 12 (2020)
