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Auteur Bo XIONG |
Documents disponibles écrits par cet auteur (2)
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Integrated Transcriptome Analyses Revealed Key Target Genes in Mouse Models of Autism / Weicheng DUAN in Autism Research, 13-3 (March 2020)
[article]
Titre : Integrated Transcriptome Analyses Revealed Key Target Genes in Mouse Models of Autism Type de document : Texte imprimé et/ou numérique Auteurs : Weicheng DUAN, Auteur ; Kang WANG, Auteur ; Yijie DUAN, Auteur ; Xufeng CHU, Auteur ; Ruoyun MA, Auteur ; Ping HU, Auteur ; Bo XIONG, Auteur Article en page(s) : p.352-368 Langues : Anglais (eng) Mots-clés : PPI network autism gene regulation integrated analysis synaptic transmission transcriptome Index. décimale : PER Périodiques Résumé : Genetic mutations are the major pathogenic factor of Autism Spectrum Disorder (ASD). In recent years, more and more ASD risk genes have been revealed, among which there are a group of transcriptional regulators. Considering the similarity of the core clinical phenotypes, it is possible that these different factors may regulate the expression levels of certain key targets. Identification of these targets could facilitate the understanding of the etiology and developing of novel diagnostic and therapeutic methods. Therefore, we performed integrated transcriptome analyses of RNA-Seq and microarray data in multiple ASD mouse models and identified a number of common downstream genes in various brain regions, many of which are related to the structure and function of the synapse components or drug addiction. We then established protein-protein interaction networks of the overlapped targets and isolated the hub genes by 11 algorithms based on the topological structure of the networks, including Sdc4, Vegfa, and Cp in the Cortex-Adult subgroup, Gria1 in the Cortex-Juvenile subgroup, and Kdr, S1pr1, Ubc, Grm2, Grin2b, Nrxn1, Pdyn, Grin3a, Itgam, Grin2a, Gabra2, and Camk4 in the Hippocampus-Adult subgroup, many of which have been associated with ASD in previous studies. Finally, we cross compared our results with human brain transcriptional data sets and verified several key candidates, which may play important role in the pathology process of ASD, including SDC4, CP, S1PR1, UBC, PDYN, GRIN2A, GABRA2, and CAMK4. In summary, by integrated bioinformatics analysis, we have identified a series of potentially important molecules for future ASD research. Autism Res 2020, 13: 352-368. (c) 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Abnormal transcriptional regulation accounts for a significant portion of Autism Spectrum Disorder. In this study, we performed transcriptome analyses of mouse models to identify common downstream targets of transcriptional regulators involved in ASD. We identified several recurrent target genes that are close related to the common pathological process of ASD, including SDC4, CP, S1PR1, UBC, PDYN, GRM2, NRXN1, GRIN3A, ITGAM, GRIN2A, GABRA2, and CAMK4. These results provide potentially important targets for understanding the molecular mechanism of ASD. En ligne : http://dx.doi.org/10.1002/aur.2240 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=421
in Autism Research > 13-3 (March 2020) . - p.352-368[article] Integrated Transcriptome Analyses Revealed Key Target Genes in Mouse Models of Autism [Texte imprimé et/ou numérique] / Weicheng DUAN, Auteur ; Kang WANG, Auteur ; Yijie DUAN, Auteur ; Xufeng CHU, Auteur ; Ruoyun MA, Auteur ; Ping HU, Auteur ; Bo XIONG, Auteur . - p.352-368.
Langues : Anglais (eng)
in Autism Research > 13-3 (March 2020) . - p.352-368
Mots-clés : PPI network autism gene regulation integrated analysis synaptic transmission transcriptome Index. décimale : PER Périodiques Résumé : Genetic mutations are the major pathogenic factor of Autism Spectrum Disorder (ASD). In recent years, more and more ASD risk genes have been revealed, among which there are a group of transcriptional regulators. Considering the similarity of the core clinical phenotypes, it is possible that these different factors may regulate the expression levels of certain key targets. Identification of these targets could facilitate the understanding of the etiology and developing of novel diagnostic and therapeutic methods. Therefore, we performed integrated transcriptome analyses of RNA-Seq and microarray data in multiple ASD mouse models and identified a number of common downstream genes in various brain regions, many of which are related to the structure and function of the synapse components or drug addiction. We then established protein-protein interaction networks of the overlapped targets and isolated the hub genes by 11 algorithms based on the topological structure of the networks, including Sdc4, Vegfa, and Cp in the Cortex-Adult subgroup, Gria1 in the Cortex-Juvenile subgroup, and Kdr, S1pr1, Ubc, Grm2, Grin2b, Nrxn1, Pdyn, Grin3a, Itgam, Grin2a, Gabra2, and Camk4 in the Hippocampus-Adult subgroup, many of which have been associated with ASD in previous studies. Finally, we cross compared our results with human brain transcriptional data sets and verified several key candidates, which may play important role in the pathology process of ASD, including SDC4, CP, S1PR1, UBC, PDYN, GRIN2A, GABRA2, and CAMK4. In summary, by integrated bioinformatics analysis, we have identified a series of potentially important molecules for future ASD research. Autism Res 2020, 13: 352-368. (c) 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Abnormal transcriptional regulation accounts for a significant portion of Autism Spectrum Disorder. In this study, we performed transcriptome analyses of mouse models to identify common downstream targets of transcriptional regulators involved in ASD. We identified several recurrent target genes that are close related to the common pathological process of ASD, including SDC4, CP, S1PR1, UBC, PDYN, GRM2, NRXN1, GRIN3A, ITGAM, GRIN2A, GABRA2, and CAMK4. These results provide potentially important targets for understanding the molecular mechanism of ASD. En ligne : http://dx.doi.org/10.1002/aur.2240 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=421 Knockout of tanc2 causes autism-like behavior and sleep disturbance in zebrafish / Fei LONG in Autism Research, 16-3 (March 2023)
[article]
Titre : Knockout of tanc2 causes autism-like behavior and sleep disturbance in zebrafish Type de document : Texte imprimé et/ou numérique Auteurs : Fei LONG, Auteur ; Jing ZHENG, Auteur ; Jiayi ZHOU, Auteur ; Ping HU, Auteur ; Bo XIONG, Auteur Article en page(s) : p.524-534 Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Abstract Tanc2 is a large multi-domain postsynaptic scaffold protein mainly expressed in the brain. In humans, tanc2 mutations have been associated with autism spectrum disorder (ASD) and other related neurodevelopmental disorders. However, the role of tanc2 in neurodevelopment and in controlling behaviors are not fully understood. Here, we generated and characterized a tanc2 knockout allele in zebrafish. Loss of tanc2 increases the larval brain size and body length by promoting proliferation and inhibiting apoptosis. We observed that the glutamatergic neuron population is significantly increased in tanc2 mutants while the GABAergic and the glycinergic neurons are not affected, suggesting that an excitatory/inhibitory (E/I) imbalance. Indeed, the tanc2 knockout larvae exhibited increase sleep. In adult zebrafish, the mutants display anxiolytic-behavior, reduced aggression, and impaired social preference. The alterations in these behaviors are phenotypically similar to the ASD patients carrying tanc2 mutations. Therefore, the tanc2 knockout allele could serve as a valuable model to further study the role of tanc2 in the nervous system. En ligne : https://doi.org/10.1002/aur.2880 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=498
in Autism Research > 16-3 (March 2023) . - p.524-534[article] Knockout of tanc2 causes autism-like behavior and sleep disturbance in zebrafish [Texte imprimé et/ou numérique] / Fei LONG, Auteur ; Jing ZHENG, Auteur ; Jiayi ZHOU, Auteur ; Ping HU, Auteur ; Bo XIONG, Auteur . - p.524-534.
Langues : Anglais (eng)
in Autism Research > 16-3 (March 2023) . - p.524-534
Index. décimale : PER Périodiques Résumé : Abstract Tanc2 is a large multi-domain postsynaptic scaffold protein mainly expressed in the brain. In humans, tanc2 mutations have been associated with autism spectrum disorder (ASD) and other related neurodevelopmental disorders. However, the role of tanc2 in neurodevelopment and in controlling behaviors are not fully understood. Here, we generated and characterized a tanc2 knockout allele in zebrafish. Loss of tanc2 increases the larval brain size and body length by promoting proliferation and inhibiting apoptosis. We observed that the glutamatergic neuron population is significantly increased in tanc2 mutants while the GABAergic and the glycinergic neurons are not affected, suggesting that an excitatory/inhibitory (E/I) imbalance. Indeed, the tanc2 knockout larvae exhibited increase sleep. In adult zebrafish, the mutants display anxiolytic-behavior, reduced aggression, and impaired social preference. The alterations in these behaviors are phenotypically similar to the ASD patients carrying tanc2 mutations. Therefore, the tanc2 knockout allele could serve as a valuable model to further study the role of tanc2 in the nervous system. En ligne : https://doi.org/10.1002/aur.2880 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=498