Dietary Intake and Plasma Levels of Choline and Betaine in Children with Autism Spectrum Disorders / Joanna C. HAMLIN in Autism Research and Treatment, (January 2014)  

Public ISBD [article]  inAutism Research and Treatment > (January 2014) . - 7 p. Titre : Dietary Intake and Plasma Levels of Choline and Betaine in Children with Autism Spectrum Disorders Type de document : Texte imprimé et/ou numérique Auteurs : Joanna C. HAMLIN, Auteur ; Margaret PAULY, Auteur ; Stepan MELNYK, Auteur ; Oleksandra PAVLIV, Auteur ; William STARRETT, Auteur ; Tina A. CROOK, Auteur ; S. Jill JAMES, Auteur Année de publication : 2014 Article en page(s) : 7 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Abnormalities in folate-dependent one-carbon metabolism have been reported in many children with autism. Because inadequate choline and betaine can negatively affect folate metabolism and in turn downstream methylation and antioxidant capacity, we sought to determine whether dietary intake of choline and betaine in children with autism was adequate to meet nutritional needs based on national recommendations. Three-day food records were analyzed for 288 children with autism (ASDs) who participated in the national Autism Intervention Research Network for Physical Health (AIR-P) Study on Diet and Nutrition in children with autism. Plasma concentrations of choline and betaine were measured in a subgroup of 35 children with ASDs and 32 age-matched control children. The results indicated that 60–93% of children with ASDs were consuming less than the recommended Adequate Intake (AI) for choline. Strong positive correlations were found between dietary intake and plasma concentrations of choline and betaine in autistic children as well as lower plasma concentrations compared to the control group. We conclude that choline and betaine intake is inadequate in a significant subgroup of children with ASDs and is reflected in lower plasma levels. Inadequate intake of choline and betaine may contribute to the metabolic abnormalities observed in many children with autism and warrants attention in nutritional counseling. En ligne : http://dx.doi.org/10.1155/2013/578429 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=228 [article] Dietary Intake and Plasma Levels of Choline and Betaine in Children with Autism Spectrum Disorders [Texte imprimé et/ou numérique] / Joanna C. HAMLIN, Auteur ; Margaret PAULY, Auteur ; Stepan MELNYK, Auteur ; Oleksandra PAVLIV, Auteur ; William STARRETT, Auteur ; Tina A. CROOK, Auteur ; S. Jill JAMES, Auteur . - 2014 . - 7 p. Langues : Anglais (eng) in Autism Research and Treatment > (January 2014) . - 7 p. Index. décimale : PER Périodiques Résumé : Abnormalities in folate-dependent one-carbon metabolism have been reported in many children with autism. Because inadequate choline and betaine can negatively affect folate metabolism and in turn downstream methylation and antioxidant capacity, we sought to determine whether dietary intake of choline and betaine in children with autism was adequate to meet nutritional needs based on national recommendations. Three-day food records were analyzed for 288 children with autism (ASDs) who participated in the national Autism Intervention Research Network for Physical Health (AIR-P) Study on Diet and Nutrition in children with autism. Plasma concentrations of choline and betaine were measured in a subgroup of 35 children with ASDs and 32 age-matched control children. The results indicated that 60–93% of children with ASDs were consuming less than the recommended Adequate Intake (AI) for choline. Strong positive correlations were found between dietary intake and plasma concentrations of choline and betaine in autistic children as well as lower plasma concentrations compared to the control group. We conclude that choline and betaine intake is inadequate in a significant subgroup of children with ASDs and is reflected in lower plasma levels. Inadequate intake of choline and betaine may contribute to the metabolic abnormalities observed in many children with autism and warrants attention in nutritional counseling. En ligne : http://dx.doi.org/10.1155/2013/578429 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=228

Effectiveness of Methylcobalamin and Folinic Acid Treatment on Adaptive Behavior in Children with Autistic Disorder Is Related to Glutathione Redox Status / Richard E. FRYE in Autism Research and Treatment, (November 2013)  

Public ISBD [article]  inAutism Research and Treatment > (November 2013) . - 9 p. Titre : Effectiveness of Methylcobalamin and Folinic Acid Treatment on Adaptive Behavior in Children with Autistic Disorder Is Related to Glutathione Redox Status Type de document : Texte imprimé et/ou numérique Auteurs : Richard E. FRYE, Auteur ; Stepan MELNYK, Auteur ; George J. FUCHS, Auteur ; Tyra REID, Auteur ; Stefanie JERNIGAN, Auteur ; Oleksandra PAVLIV, Auteur ; Amanda HUBANKS, Auteur ; David W. GAYLOR, Auteur ; Laura WALTERS, Auteur ; S. Jill JAMES, Auteur Année de publication : 2013 Article en page(s) : 9 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Treatments targeting metabolic abnormalities in children with autism are limited. Previously we reported that a nutritional treatment significantly improved glutathione metabolism in children with autistic disorder. In this study we evaluated changes in adaptive behaviors in this cohort and determined whether such changes are related to changes in glutathione metabolism. Thirty-seven children diagnosed with autistic disorder and abnormal glutathione and methylation metabolism were treated with twice weekly 75?µg/Kg methylcobalamin and twice daily 400?µg folinic acid for 3 months in an open-label fashion. The Vineland Adaptive Behavior Scale (VABS) and glutathione redox metabolites were measured at baseline and at the end of the treatment period. Over the treatment period, all VABS subscales significantly improved with an average effect size of 0.59, and an average improvement in skills of 7.7 months. A greater improvement in glutathione redox status was associated with a greater improvement in expressive communication, personal and domestic daily living skills, and interpersonal, play-leisure, and coping social skills. Age, gender, and history of regression did not influence treatment response. The significant behavioral improvements observed and the relationship between these improvements to glutathione redox status suggest that nutritional interventions targeting redox metabolism may benefit some children with autism. En ligne : http://dx.doi.org/10.1155/2013/609705 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=228 [article] Effectiveness of Methylcobalamin and Folinic Acid Treatment on Adaptive Behavior in Children with Autistic Disorder Is Related to Glutathione Redox Status [Texte imprimé et/ou numérique] / Richard E. FRYE, Auteur ; Stepan MELNYK, Auteur ; George J. FUCHS, Auteur ; Tyra REID, Auteur ; Stefanie JERNIGAN, Auteur ; Oleksandra PAVLIV, Auteur ; Amanda HUBANKS, Auteur ; David W. GAYLOR, Auteur ; Laura WALTERS, Auteur ; S. Jill JAMES, Auteur . - 2013 . - 9 p. Langues : Anglais (eng) in Autism Research and Treatment > (November 2013) . - 9 p. Index. décimale : PER Périodiques Résumé : Treatments targeting metabolic abnormalities in children with autism are limited. Previously we reported that a nutritional treatment significantly improved glutathione metabolism in children with autistic disorder. In this study we evaluated changes in adaptive behaviors in this cohort and determined whether such changes are related to changes in glutathione metabolism. Thirty-seven children diagnosed with autistic disorder and abnormal glutathione and methylation metabolism were treated with twice weekly 75?µg/Kg methylcobalamin and twice daily 400?µg folinic acid for 3 months in an open-label fashion. The Vineland Adaptive Behavior Scale (VABS) and glutathione redox metabolites were measured at baseline and at the end of the treatment period. Over the treatment period, all VABS subscales significantly improved with an average effect size of 0.59, and an average improvement in skills of 7.7 months. A greater improvement in glutathione redox status was associated with a greater improvement in expressive communication, personal and domestic daily living skills, and interpersonal, play-leisure, and coping social skills. Age, gender, and history of regression did not influence treatment response. The significant behavioral improvements observed and the relationship between these improvements to glutathione redox status suggest that nutritional interventions targeting redox metabolism may benefit some children with autism. En ligne : http://dx.doi.org/10.1155/2013/609705 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=228

Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism / Shannon ROSE in Autism Research and Treatment, (September 2011)  

Public ISBD [article]  inAutism Research and Treatment > (September 2011) . - 10 p. Titre : Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism Type de document : Texte imprimé et/ou numérique Auteurs : Shannon ROSE, Auteur ; Stepan MELNYK, Auteur ; Timothy A. TRUSTY, Auteur ; Oleksandra PAVLIV, Auteur ; Lisa SEIDEL, Auteur ; Jingyun LI, Auteur ; Todd NICK, Auteur ; S. Jill JAMES, Auteur Année de publication : 2011 Article en page(s) : 10 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : The modulation of the redox microenvironment is an important regulator of immune cell activation and proliferation. To investigate immune cell redox status in autism we quantified the intracellular glutathione redox couple (GSH/GSSG) in resting peripheral blood mononuclear cells (PBMCs), activated monocytes and CD4 T cells and the extracellular cysteine/cystine redox couple in the plasma from 43 children with autism and 41 age-matched control children. Resting PBMCs and activated monocytes from children with autism exhibited significantly higher oxidized glutathione (GSSG) and percent oxidized glutathione equivalents and decreased glutathione redox status (GSH/GSSG). In activated CD4 T cells from children with autism, the percent oxidized glutathione equivalents were similarly increased, and GSH and GSH/GSSG were decreased. In the plasma, both glutathione and cysteine redox ratios were decreased in autistic compared to control children. Consistent with decreased intracellular and extracellular redox status, generation of free radicals was significantly elevated in lymphocytes from the autistic children. These data indicate primary immune cells from autistic children have a more oxidized intracellular and extracellular microenvironment and a deficit in glutathione-mediated redox/antioxidant capacity compared to control children. These results suggest that the loss of glutathione redox homeostasis and chronic oxidative stress may contribute to immune dysregulation in autism. En ligne : http://dx.doi.org/10.1155/2012/986519 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=149 [article] Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism [Texte imprimé et/ou numérique] / Shannon ROSE, Auteur ; Stepan MELNYK, Auteur ; Timothy A. TRUSTY, Auteur ; Oleksandra PAVLIV, Auteur ; Lisa SEIDEL, Auteur ; Jingyun LI, Auteur ; Todd NICK, Auteur ; S. Jill JAMES, Auteur . - 2011 . - 10 p. Langues : Anglais (eng) in Autism Research and Treatment > (September 2011) . - 10 p. Index. décimale : PER Périodiques Résumé : The modulation of the redox microenvironment is an important regulator of immune cell activation and proliferation. To investigate immune cell redox status in autism we quantified the intracellular glutathione redox couple (GSH/GSSG) in resting peripheral blood mononuclear cells (PBMCs), activated monocytes and CD4 T cells and the extracellular cysteine/cystine redox couple in the plasma from 43 children with autism and 41 age-matched control children. Resting PBMCs and activated monocytes from children with autism exhibited significantly higher oxidized glutathione (GSSG) and percent oxidized glutathione equivalents and decreased glutathione redox status (GSH/GSSG). In activated CD4 T cells from children with autism, the percent oxidized glutathione equivalents were similarly increased, and GSH and GSH/GSSG were decreased. In the plasma, both glutathione and cysteine redox ratios were decreased in autistic compared to control children. Consistent with decreased intracellular and extracellular redox status, generation of free radicals was significantly elevated in lymphocytes from the autistic children. These data indicate primary immune cells from autistic children have a more oxidized intracellular and extracellular microenvironment and a deficit in glutathione-mediated redox/antioxidant capacity compared to control children. These results suggest that the loss of glutathione redox homeostasis and chronic oxidative stress may contribute to immune dysregulation in autism. En ligne : http://dx.doi.org/10.1155/2012/986519 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=149

Maternal metabolic profile predicts high or low risk of an autism pregnancy outcome / Kathryn HOLLOWOOD in Research in Autism Spectrum Disorders, 56 (December 2018)  

Public ISBD [article]  inResearch in Autism Spectrum Disorders > 56 (December 2018) . - p.72-82 Titre : Maternal metabolic profile predicts high or low risk of an autism pregnancy outcome Type de document : Texte imprimé et/ou numérique Auteurs : Kathryn HOLLOWOOD, Auteur ; Stepan MELNYK, Auteur ; Oleksandra PAVLIV, Auteur ; Teresa EVANS, Auteur ; Ashley SIDES, Auteur ; Rebecca J. SCHMIDT, Auteur ; Irva HERTZ-PICCIOTTO, Auteur ; William ELMS, Auteur ; Elizabeth GUERRERO, Auteur ; Uwe KRUGER, Auteur ; Juergen HAHN, Auteur ; S. Jill JAMES, Auteur Article en page(s) : p.72-82 Langues : Anglais (eng) Mots-clés : Autism  Pregnancy  Metabolic profile  Folate  Transmethylation  Transsulfuration  Fisher discriminant analysis Index. décimale : PER Périodiques Résumé : Background Currently there is no test for pregnant mothers that can predict the probability of having a child that will be diagnosed with autism spectrum disorder (ASD). Recent estimates indicate that if a mother has previously had a child with ASD, the risk of having a second child with ASD is ?18.7% (High Risk) whereas the risk of ASD in the general population is ?1.7% (Low Risk). Methods In this study, metabolites of the folate-dependent transmethylation and transsulfuration biochemical pathways of pregnant mothers were measured to determine whether or not the risk of having a child with autism could be predicted by her metabolic profile. Pregnant mothers who have had a child with autism before were separated into two groups based on the diagnosis of their child whether the child had autism (ASD) or not (TD). Then these mothers were compared to a group of control mothers who have not had a child with autism before. A total of 107 mothers were in the High Risk category and 25 mothers in the Low Risk category. The High Risk category was further separated into 29 mothers in the ASD group and 78 mothers in the TD group. Results The metabolic results indicated that among High Risk mothers, it was not possible to predict an autism pregnancy outcome. However, the metabolic profile was able to predict with approximately 90% sensitivity and specificity whether a mother fell into the High Risk group (18.7% risk) or Low Risk group (1.7% risk). Conclusions Based upon these measurements it is not possible to determine during a pregnancy if a child will be diagnosed with ASD by age 3. However, differences in the folate-dependent transmethylation and transsulfuration metabolites are indicative of the risk level (High Risk of 18.7% vs. Low Risk of 1.7%) of the mother for having a child with ASD. En ligne : https://doi.org/10.1016/j.rasd.2018.09.003 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=369 [article] Maternal metabolic profile predicts high or low risk of an autism pregnancy outcome [Texte imprimé et/ou numérique] / Kathryn HOLLOWOOD, Auteur ; Stepan MELNYK, Auteur ; Oleksandra PAVLIV, Auteur ; Teresa EVANS, Auteur ; Ashley SIDES, Auteur ; Rebecca J. SCHMIDT, Auteur ; Irva HERTZ-PICCIOTTO, Auteur ; William ELMS, Auteur ; Elizabeth GUERRERO, Auteur ; Uwe KRUGER, Auteur ; Juergen HAHN, Auteur ; S. Jill JAMES, Auteur . - p.72-82. Langues : Anglais (eng) in Research in Autism Spectrum Disorders > 56 (December 2018) . - p.72-82 Mots-clés : Autism  Pregnancy  Metabolic profile  Folate  Transmethylation  Transsulfuration  Fisher discriminant analysis Index. décimale : PER Périodiques Résumé : Background Currently there is no test for pregnant mothers that can predict the probability of having a child that will be diagnosed with autism spectrum disorder (ASD). Recent estimates indicate that if a mother has previously had a child with ASD, the risk of having a second child with ASD is ?18.7% (High Risk) whereas the risk of ASD in the general population is ?1.7% (Low Risk). Methods In this study, metabolites of the folate-dependent transmethylation and transsulfuration biochemical pathways of pregnant mothers were measured to determine whether or not the risk of having a child with autism could be predicted by her metabolic profile. Pregnant mothers who have had a child with autism before were separated into two groups based on the diagnosis of their child whether the child had autism (ASD) or not (TD). Then these mothers were compared to a group of control mothers who have not had a child with autism before. A total of 107 mothers were in the High Risk category and 25 mothers in the Low Risk category. The High Risk category was further separated into 29 mothers in the ASD group and 78 mothers in the TD group. Results The metabolic results indicated that among High Risk mothers, it was not possible to predict an autism pregnancy outcome. However, the metabolic profile was able to predict with approximately 90% sensitivity and specificity whether a mother fell into the High Risk group (18.7% risk) or Low Risk group (1.7% risk). Conclusions Based upon these measurements it is not possible to determine during a pregnancy if a child will be diagnosed with ASD by age 3. However, differences in the folate-dependent transmethylation and transsulfuration metabolites are indicative of the risk level (High Risk of 18.7% vs. Low Risk of 1.7%) of the mother for having a child with ASD. En ligne : https://doi.org/10.1016/j.rasd.2018.09.003 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=369

Metabolic Imbalance Associated with Methylation Dysregulation and Oxidative Damage in Children with Autism / Stepan MELNYK in Journal of Autism and Developmental Disorders, 42-3 (March 2012)  

Public ISBD [article]  inJournal of Autism and Developmental Disorders > 42-3 (March 2012) . - p.367-377 Titre : Metabolic Imbalance Associated with Methylation Dysregulation and Oxidative Damage in Children with Autism Type de document : Texte imprimé et/ou numérique Auteurs : Stepan MELNYK, Auteur ; George J. FUCHS, Auteur ; Eldon SCHULZ, Auteur ; Maya LOPEZ, Auteur ; Stephen G. KAHLER, Auteur ; Jill J. FUSSELL, Auteur ; Jayne BELLANDO, Auteur ; Oleksandra PAVLIV, Auteur ; Shannon ROSE, Auteur ; Lisa SEIDEL, Auteur ; David W. GAYLOR, Auteur ; S. Jill JAMES, Auteur Année de publication : 2012 Article en page(s) : p.367-377 Langues : Anglais (eng) Mots-clés : Autism  Oxidative stress  Metabolic  Epigenetics  Glutathione  DNA methylation Index. décimale : PER Périodiques Résumé : Oxidative stress and abnormal DNA methylation have been implicated in the pathophysiology of autism. We investigated the dynamics of an integrated metabolic pathway essential for cellular antioxidant and methylation capacity in 68 children with autism, 54 age-matched control children and 40 unaffected siblings. The metabolic profile of unaffected siblings differed significantly from case siblings but not from controls. Oxidative protein/DNA damage and DNA hypomethylation (epigenetic alteration) were found in autistic children but not paired siblings or controls. These data indicate that the deficit in antioxidant and methylation capacity is specific for autism and may promote cellular damage and altered epigenetic gene expression. Further, these results suggest a plausible mechanism by which pro-oxidant environmental stressors may modulate genetic predisposition to autism. En ligne : http://dx.doi.org/10.1007/s10803-011-1260-7 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=152 [article] Metabolic Imbalance Associated with Methylation Dysregulation and Oxidative Damage in Children with Autism [Texte imprimé et/ou numérique] / Stepan MELNYK, Auteur ; George J. FUCHS, Auteur ; Eldon SCHULZ, Auteur ; Maya LOPEZ, Auteur ; Stephen G. KAHLER, Auteur ; Jill J. FUSSELL, Auteur ; Jayne BELLANDO, Auteur ; Oleksandra PAVLIV, Auteur ; Shannon ROSE, Auteur ; Lisa SEIDEL, Auteur ; David W. GAYLOR, Auteur ; S. Jill JAMES, Auteur . - 2012 . - p.367-377. Langues : Anglais (eng) in Journal of Autism and Developmental Disorders > 42-3 (March 2012) . - p.367-377 Mots-clés : Autism  Oxidative stress  Metabolic  Epigenetics  Glutathione  DNA methylation Index. décimale : PER Périodiques Résumé : Oxidative stress and abnormal DNA methylation have been implicated in the pathophysiology of autism. We investigated the dynamics of an integrated metabolic pathway essential for cellular antioxidant and methylation capacity in 68 children with autism, 54 age-matched control children and 40 unaffected siblings. The metabolic profile of unaffected siblings differed significantly from case siblings but not from controls. Oxidative protein/DNA damage and DNA hypomethylation (epigenetic alteration) were found in autistic children but not paired siblings or controls. These data indicate that the deficit in antioxidant and methylation capacity is specific for autism and may promote cellular damage and altered epigenetic gene expression. Further, these results suggest a plausible mechanism by which pro-oxidant environmental stressors may modulate genetic predisposition to autism. En ligne : http://dx.doi.org/10.1007/s10803-011-1260-7 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=152

---

1 (1 - 5 / 5)