Identification of an age-dependent biomarker signature in children and adolescents with autism spectrum disorders / Jordan M. RAMSEY in Molecular Autism, (August 2013)  

Public ISBD [article]  inMolecular Autism > (August 2013) . - 18 p. Titre : Identification of an age-dependent biomarker signature in children and adolescents with autism spectrum disorders Type de document : Texte imprimé et/ou numérique Auteurs : Jordan M. RAMSEY, Auteur ; Paul C. GUEST, Auteur ; Jantine A.C. BROEK, Auteur ; Jeffrey GLENNON, Auteur ; Nanda N. ROMMELSE, Auteur ; Barbara FRANKE, Auteur ; Hassan RAHMOUNE, Auteur ; Jan K. BUITELAAR, Auteur ; Sabine BAHN, Auteur Année de publication : 2013 Article en page(s) : 18 p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Background Autism spectrum disorders (ASDs) are neurodevelopmental conditions with symptoms manifesting before the age of 3, generally persisting throughout life and affecting social development and communication. Here, we have investigated changes in protein biomarkers in blood during childhood and adolescent development. Methods We carried out a multiplex immunoassay profiling analysis of serum samples from 37 individuals with a diagnosis of ASD and their matched, non-affected siblings, aged between 4 and 18 years, to identify molecular pathways affected over the course of ASDs. Results This analysis revealed age-dependent differences in the levels of 12 proteins involved in inflammation, growth and hormonal signaling. Conclusions These deviations in age-related molecular trajectories provide further insight into the progression and pathophysiology of the disorder and, if replicated, may contribute to better classification of ASD individuals, as well as to improved treatment and prognosis. The results also underline the importance of stratifying and analyzing samples by age, especially in ASD and potentially other developmental disorders. En ligne : http://dx.doi.org/10.1186/2040-2392-4-27 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=211 [article] Identification of an age-dependent biomarker signature in children and adolescents with autism spectrum disorders [Texte imprimé et/ou numérique] / Jordan M. RAMSEY, Auteur ; Paul C. GUEST, Auteur ; Jantine A.C. BROEK, Auteur ; Jeffrey GLENNON, Auteur ; Nanda N. ROMMELSE, Auteur ; Barbara FRANKE, Auteur ; Hassan RAHMOUNE, Auteur ; Jan K. BUITELAAR, Auteur ; Sabine BAHN, Auteur . - 2013 . - 18 p. Langues : Anglais (eng) in Molecular Autism > (August 2013) . - 18 p. Index. décimale : PER Périodiques Résumé : Background Autism spectrum disorders (ASDs) are neurodevelopmental conditions with symptoms manifesting before the age of 3, generally persisting throughout life and affecting social development and communication. Here, we have investigated changes in protein biomarkers in blood during childhood and adolescent development. Methods We carried out a multiplex immunoassay profiling analysis of serum samples from 37 individuals with a diagnosis of ASD and their matched, non-affected siblings, aged between 4 and 18 years, to identify molecular pathways affected over the course of ASDs. Results This analysis revealed age-dependent differences in the levels of 12 proteins involved in inflammation, growth and hormonal signaling. Conclusions These deviations in age-related molecular trajectories provide further insight into the progression and pathophysiology of the disorder and, if replicated, may contribute to better classification of ASD individuals, as well as to improved treatment and prognosis. The results also underline the importance of stratifying and analyzing samples by age, especially in ASD and potentially other developmental disorders. En ligne : http://dx.doi.org/10.1186/2040-2392-4-27 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=211

Integrative proteomic analysis of the NMDA NR1 knockdown mouse model reveals effects on central and peripheral pathways associated with schizophrenia and autism spectrum disorders / Hendrik WESSELING in Molecular Autism, (July 2014)  

Public ISBD [article]  inMolecular Autism > (July 2014) . - p.1-17 Titre : Integrative proteomic analysis of the NMDA NR1 knockdown mouse model reveals effects on central and peripheral pathways associated with schizophrenia and autism spectrum disorders Type de document : Texte imprimé et/ou numérique Auteurs : Hendrik WESSELING, Auteur ; Paul C. GUEST, Auteur ; Chi-Ming LEE, Auteur ; Erik HF WONG, Auteur ; Hassan RAHMOUNE, Auteur ; Sabine BAHN, Auteur Article en page(s) : p.1-17 Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Over the last decade, the transgenic N-methyl-D-aspartate receptor (NMDAR) NR1-knockdown mouse (NR1neo?/?) has been investigated as a glutamate hypofunction model for schizophrenia. Recent research has now revealed that the model also recapitulates cognitive and negative symptoms in the continuum of other psychiatric diseases, particularly autism spectrum disorders (ASD). As previous studies have mostly focussed on behavioural readouts, a molecular characterisation of this model will help to identify novel biomarkers or potential drug targets. En ligne : http://dx.doi.org/10.1186/2040-2392-5-38 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=276 [article] Integrative proteomic analysis of the NMDA NR1 knockdown mouse model reveals effects on central and peripheral pathways associated with schizophrenia and autism spectrum disorders [Texte imprimé et/ou numérique] / Hendrik WESSELING, Auteur ; Paul C. GUEST, Auteur ; Chi-Ming LEE, Auteur ; Erik HF WONG, Auteur ; Hassan RAHMOUNE, Auteur ; Sabine BAHN, Auteur . - p.1-17. Langues : Anglais (eng) in Molecular Autism > (July 2014) . - p.1-17 Index. décimale : PER Périodiques Résumé : Over the last decade, the transgenic N-methyl-D-aspartate receptor (NMDAR) NR1-knockdown mouse (NR1neo?/?) has been investigated as a glutamate hypofunction model for schizophrenia. Recent research has now revealed that the model also recapitulates cognitive and negative symptoms in the continuum of other psychiatric diseases, particularly autism spectrum disorders (ASD). As previous studies have mostly focussed on behavioural readouts, a molecular characterisation of this model will help to identify novel biomarkers or potential drug targets. En ligne : http://dx.doi.org/10.1186/2040-2392-5-38 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=276

Proteomic analysis of post mortem brain tissue from autism patients: evidence for opposite changes in prefrontal cortex and cerebellum in synaptic connectivity-related proteins / Jantine A.C. BROEK in Molecular Autism, (July 2014)  

Public ISBD [article]  inMolecular Autism > (July 2014) . - p.1-8 Titre : Proteomic analysis of post mortem brain tissue from autism patients: evidence for opposite changes in prefrontal cortex and cerebellum in synaptic connectivity-related proteins Type de document : Texte imprimé et/ou numérique Auteurs : Jantine A.C. BROEK, Auteur ; Paul C. GUEST, Auteur ; Hassan RAHMOUNE, Auteur ; Sabine BAHN, Auteur Article en page(s) : p.1-8 Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : Autism is a neurodevelopmental disorder characterized by impaired language, communication and social skills. Although genetic studies have been carried out in this field, none of the genes identified have led to an explanation of the underlying causes. Here, we have investigated molecular alterations by proteomic profiling of post mortem brain samples from autism patients and controls. The analysis focussed on prefrontal cortex and cerebellum as previous studies have found that these two brain regions are structurally and functionally connected, and they have been implicated in autism. En ligne : http://dx.doi.org/10.1186/2040-2392-5-41 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=276 [article] Proteomic analysis of post mortem brain tissue from autism patients: evidence for opposite changes in prefrontal cortex and cerebellum in synaptic connectivity-related proteins [Texte imprimé et/ou numérique] / Jantine A.C. BROEK, Auteur ; Paul C. GUEST, Auteur ; Hassan RAHMOUNE, Auteur ; Sabine BAHN, Auteur . - p.1-8. Langues : Anglais (eng) in Molecular Autism > (July 2014) . - p.1-8 Index. décimale : PER Périodiques Résumé : Autism is a neurodevelopmental disorder characterized by impaired language, communication and social skills. Although genetic studies have been carried out in this field, none of the genes identified have led to an explanation of the underlying causes. Here, we have investigated molecular alterations by proteomic profiling of post mortem brain samples from autism patients and controls. The analysis focussed on prefrontal cortex and cerebellum as previous studies have found that these two brain regions are structurally and functionally connected, and they have been implicated in autism. En ligne : http://dx.doi.org/10.1186/2040-2392-5-41 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=276

Serum proteomic analysis identifies sex-specific differences in lipid metabolism and inflammation profiles in adults diagnosed with Asperger syndrome / Hannah STEEB in Molecular Autism, (January 2014)  

Public ISBD [article]  inMolecular Autism > (January 2014) Titre : Serum proteomic analysis identifies sex-specific differences in lipid metabolism and inflammation profiles in adults diagnosed with Asperger syndrome Type de document : Texte imprimé et/ou numérique Auteurs : Hannah STEEB, Auteur ; Jordan RAMSEY, Auteur ; Paul GUEST, Auteur ; Pawel STOCKI, Auteur ; Jason COOPER, Auteur ; Hassan RAHMOUNE, Auteur ; Erin INGUDOMNUKUL, Auteur ; Bonnie AUYEUNG, Auteur ; Liliana RUTA, Auteur ; Simon BARON-COHEN, Auteur ; Sabine BAHN, Auteur Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : The higher prevalence of Asperger Syndrome (AS) and other autism spectrum conditions in males has been known for many years. However, recent multiplex immunoassay profiling studies have shown that males and females with AS have distinct proteomic changes in serum. Here, we analysed sera from adults diagnosed with AS (males=14, females=16) and controls (males=13, females=16) not on medication at the time of sample collection, using a combination of multiplex immunoassay and shotgun label-free liquid chromatography mass spectrometry (LC-MSE). The main objective was to identify sex-specific serum protein changes associated with AS. Multiplex immunoassay profiling led to identification of 16 proteins that were significantly altered in AS individuals in a sex-specific manner. Three of these proteins were altered in females (ADIPO, IgA, APOA1), seven were changed in males (BMP6, CTGF, ICAM1, IL-12p70, IL-16, TF, TNF-alpha) and six were changed in both sexes but in opposite directions (CHGA, EPO, IL-3, TENA, PAP, SHBG). Shotgun LC-MSE profiling led to identification of 13 serum proteins which had significant sex-specific changes in the AS group and, of these, 12 were altered in females (APOC2, APOE, ARMC3, CLC4K, FETUB, GLCE, MRRP1, PTPA, RN149, TLE1, TRIPB, ZC3HE) and one protein was altered in males (RGPD4). The free androgen index in females with AS showed an increased ratio of 1.63 compared to controls.CONCLUSION:Taken together, the serum multiplex immunoassay and shotgun LC-MSE profiling results indicate that adult females with AS had alterations in proteins involved mostly in lipid transport and metabolism pathways, while adult males with AS showed changes predominantly in inflammation signalling. These results provide further evidence that the search for biomarkers or novel drug targets in AS may require stratification into male and female subgroups, and could lead to the development of novel targeted treatment approaches. En ligne : http://dx.doi.org/10.1186/2040-2392-5-4 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=227 [article] Serum proteomic analysis identifies sex-specific differences in lipid metabolism and inflammation profiles in adults diagnosed with Asperger syndrome [Texte imprimé et/ou numérique] / Hannah STEEB, Auteur ; Jordan RAMSEY, Auteur ; Paul GUEST, Auteur ; Pawel STOCKI, Auteur ; Jason COOPER, Auteur ; Hassan RAHMOUNE, Auteur ; Erin INGUDOMNUKUL, Auteur ; Bonnie AUYEUNG, Auteur ; Liliana RUTA, Auteur ; Simon BARON-COHEN, Auteur ; Sabine BAHN, Auteur. Langues : Anglais (eng) in Molecular Autism > (January 2014) Index. décimale : PER Périodiques Résumé : The higher prevalence of Asperger Syndrome (AS) and other autism spectrum conditions in males has been known for many years. However, recent multiplex immunoassay profiling studies have shown that males and females with AS have distinct proteomic changes in serum. Here, we analysed sera from adults diagnosed with AS (males=14, females=16) and controls (males=13, females=16) not on medication at the time of sample collection, using a combination of multiplex immunoassay and shotgun label-free liquid chromatography mass spectrometry (LC-MSE). The main objective was to identify sex-specific serum protein changes associated with AS. Multiplex immunoassay profiling led to identification of 16 proteins that were significantly altered in AS individuals in a sex-specific manner. Three of these proteins were altered in females (ADIPO, IgA, APOA1), seven were changed in males (BMP6, CTGF, ICAM1, IL-12p70, IL-16, TF, TNF-alpha) and six were changed in both sexes but in opposite directions (CHGA, EPO, IL-3, TENA, PAP, SHBG). Shotgun LC-MSE profiling led to identification of 13 serum proteins which had significant sex-specific changes in the AS group and, of these, 12 were altered in females (APOC2, APOE, ARMC3, CLC4K, FETUB, GLCE, MRRP1, PTPA, RN149, TLE1, TRIPB, ZC3HE) and one protein was altered in males (RGPD4). The free androgen index in females with AS showed an increased ratio of 1.63 compared to controls.CONCLUSION:Taken together, the serum multiplex immunoassay and shotgun LC-MSE profiling results indicate that adult females with AS had alterations in proteins involved mostly in lipid transport and metabolism pathways, while adult males with AS showed changes predominantly in inflammation signalling. These results provide further evidence that the search for biomarkers or novel drug targets in AS may require stratification into male and female subgroups, and could lead to the development of novel targeted treatment approaches. En ligne : http://dx.doi.org/10.1186/2040-2392-5-4 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=227

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