ADHD and autism symptoms in youth: a network analysis / L. C. FARHAT in Journal of Child Psychology and Psychiatry, 63-2 (February 2022)  

Public ISBD [article]  inJournal of Child Psychology and Psychiatry > 63-2 (February 2022) . - p.143-151 Titre : ADHD and autism symptoms in youth: a network analysis Type de document : Texte imprimé et/ou numérique Auteurs : L. C. FARHAT, Auteur ; Helena BRENTANI, Auteur ; V. H. C. DE TOLEDO, Auteur ; E. SHEPHARD, Auteur ; P. MATTOS, Auteur ; Simon BARON-COHEN, Auteur ; A. THAPAR, Auteur ; E. CASELLA, Auteur ; Guilherme V. POLANCZYK, Auteur Article en page(s) : p.143-151 Langues : Anglais (eng) Mots-clés : Attention-deficit hyperactivity disorder  autism spectrum disorder  comorbidity  network analysis  neurodevelopmental Index. décimale : PER Périodiques Résumé : BACKGROUND: Previous research investigating the overlap between attention-deficit/hyperactivity disorder (ADHD) and autism spectrum disorder (henceforth, autism) symptoms in population samples have relied on latent variable modeling in which averaged scores representing dimensions were derived from observed symptoms. There are no studies evaluating how ADHD and autism symptoms interact at the level of individual symptom items. METHODS: We aimed to address this gap by performing a network analysis on data from a school survey of children aged 6-17?years old (N?=?7,405). ADHD and autism symptoms were measured via parent-report on the Swanson, Nolan, Pelham-IV questionnaire and the Childhood Autism Spectrum test, respectively. RESULTS: A relatively low interconnectivity between ADHD and autism symptoms was found with only 10.06% of possible connections (edges) between one ADHD and one autism symptoms different than zero. Associations between ADHD and autism symptoms were significantly weaker than those between two symptoms pertaining to the same construct. Select ADHD symptoms, particularly those presenting in social contexts (e.g. 'talks excessively', 'does not wait turn'), showed moderate-to-strong associations with autism symptoms, but some were considered redundant to autism symptoms. CONCLUSIONS: The present findings indicate that individual ADHD and autism symptoms are largely segregated in accordance with diagnostic boundaries corresponding to these conditions in children and adolescents from the community. These findings could improve our clinical conceptualization of ADHD and autism and guide advancements in diagnosis and treatment. En ligne : http://dx.doi.org/10.1111/jcpp.13436 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=457 [article] ADHD and autism symptoms in youth: a network analysis [Texte imprimé et/ou numérique] / L. C. FARHAT, Auteur ; Helena BRENTANI, Auteur ; V. H. C. DE TOLEDO, Auteur ; E. SHEPHARD, Auteur ; P. MATTOS, Auteur ; Simon BARON-COHEN, Auteur ; A. THAPAR, Auteur ; E. CASELLA, Auteur ; Guilherme V. POLANCZYK, Auteur . - p.143-151. Langues : Anglais (eng) in Journal of Child Psychology and Psychiatry > 63-2 (February 2022) . - p.143-151 Mots-clés : Attention-deficit hyperactivity disorder  autism spectrum disorder  comorbidity  network analysis  neurodevelopmental Index. décimale : PER Périodiques Résumé : BACKGROUND: Previous research investigating the overlap between attention-deficit/hyperactivity disorder (ADHD) and autism spectrum disorder (henceforth, autism) symptoms in population samples have relied on latent variable modeling in which averaged scores representing dimensions were derived from observed symptoms. There are no studies evaluating how ADHD and autism symptoms interact at the level of individual symptom items. METHODS: We aimed to address this gap by performing a network analysis on data from a school survey of children aged 6-17?years old (N?=?7,405). ADHD and autism symptoms were measured via parent-report on the Swanson, Nolan, Pelham-IV questionnaire and the Childhood Autism Spectrum test, respectively. RESULTS: A relatively low interconnectivity between ADHD and autism symptoms was found with only 10.06% of possible connections (edges) between one ADHD and one autism symptoms different than zero. Associations between ADHD and autism symptoms were significantly weaker than those between two symptoms pertaining to the same construct. Select ADHD symptoms, particularly those presenting in social contexts (e.g. 'talks excessively', 'does not wait turn'), showed moderate-to-strong associations with autism symptoms, but some were considered redundant to autism symptoms. CONCLUSIONS: The present findings indicate that individual ADHD and autism symptoms are largely segregated in accordance with diagnostic boundaries corresponding to these conditions in children and adolescents from the community. These findings could improve our clinical conceptualization of ADHD and autism and guide advancements in diagnosis and treatment. En ligne : http://dx.doi.org/10.1111/jcpp.13436 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=457

An Exact Mind / Peter MYERS

Public ISBD Titre : An Exact Mind : An Artist with Asperger Syndrome Type de document : Texte imprimé et/ou numérique Auteurs : Peter MYERS, Auteur ; Sally WHEELWRIGHT, Auteur ; Simon BARON-COHEN, Auteur Editeur : Londres [Angleterre] : Jessica Kingsley Publishers Année de publication : 2004 Importance : 79 p. Présentation : ill. Format : 30cm x 24cm x 0,5cm ISBN/ISSN/EAN : 978-1-84310-032-4 Langues : Anglais (eng) Index. décimale : ART-A ART-A - Artistes Autistes Résumé : Peter Myers's intricate and ornately patterned drawings are brought together for the first time in this volume, which is the fascinating result of the collaboration of an artist and two scientists. The beautiful, complex images (included in full-page colour as well as black and white reproductions) serve as a rare window into the precision and creativity of the Asperger mind at work. Peter Myers was diagnosed with Asperger Syndrome in 1996 and his work reflects his stunning ability to plan and to organize visual information, and to embed illusions within his pictures. In explanatory text alongside the pictures, psychologists Simon Baron-Cohen and Sally Wheelright discuss the work's deep psychological significance, demonstrating in accessible language their ground-breaking systemizing theory of how the autistic mind processes information. Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=385 An Exact Mind : An Artist with Asperger Syndrome [Texte imprimé et/ou numérique] / Peter MYERS, Auteur ; Sally WHEELWRIGHT, Auteur ; Simon BARON-COHEN, Auteur . - Londres [Angleterre] : Jessica Kingsley Publishers, 2004 . - 79 p. : ill. ; 30cm x 24cm x 0,5cm. ISBN : 978-1-84310-032-4 Langues : Anglais (eng) Index. décimale : ART-A ART-A - Artistes Autistes Résumé : Peter Myers's intricate and ornately patterned drawings are brought together for the first time in this volume, which is the fascinating result of the collaboration of an artist and two scientists. The beautiful, complex images (included in full-page colour as well as black and white reproductions) serve as a rare window into the precision and creativity of the Asperger mind at work. Peter Myers was diagnosed with Asperger Syndrome in 1996 and his work reflects his stunning ability to plan and to organize visual information, and to embed illusions within his pictures. In explanatory text alongside the pictures, psychologists Simon Baron-Cohen and Sally Wheelright discuss the work's deep psychological significance, demonstrating in accessible language their ground-breaking systemizing theory of how the autistic mind processes information. Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=385

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
DOC0000209	ART-A MYE	Livre	Centre d'Information et de Documentation du CRA Rhône-Alpes	ART - Autisme et art - Autisme en images	Disponible

Les abonnés qui ont emprunté ce document ont également emprunté :

La douance et la double exceptionnalité chez l'enfant et l'adolescent	CARON, Marie-Josée
Asperger Syndrome and Anxiety	DUBIN, Nick
L'éternité dans une heure	TAMMET, Daniel
The Asperger Couple's Workbook	ASTON, Maxine
L'autisme insoupçonné	GRAND, Claire
Un anthropologue sur Mars	SACKS, Oliver

An investigation of the diet, exercise, sleep, BMI, and health outcomes of autistic adults / E. WEIR in Molecular Autism, 12 (2021)  

Public ISBD [article]  inMolecular Autism > 12 (2021) . - 31 p. Titre : An investigation of the diet, exercise, sleep, BMI, and health outcomes of autistic adults Type de document : Texte imprimé et/ou numérique Auteurs : E. WEIR, Auteur ; Carrie ALLISON, Auteur ; K. K. ONG, Auteur ; Simon BARON-COHEN, Auteur Article en page(s) : 31 p. Langues : Anglais (eng) Mots-clés : Adult outcomes  Comorbidities  Exercise  Healthcare  Nutrition  Physical health  Sleep Index. décimale : PER Périodiques Résumé : BACKGROUND: Studies of autistic children suggest that restricted eating, reduced physical activity, and sleep disorders are common; however, no studies attempt to broadly describe the diet, exercise, and sleep patterns of autistic adults or consider relationships between lifestyle behaviors and the widely reported increased risks of obesity and chronic conditions. To address this, the authors developed the largest study of lifestyle patterns of autistic adults and assessed their relationships to body mass index, health outcomes, and family history. METHODS: We administered an anonymized, online survey to n?=?2386 adults (n?=?1183 autistic) aged 16-90 years of age. We employed Fisher's exact tests and binomial logistic regression to describe diet, exercise, and sleep patterns; mediation of seizure disorders on sleep; body mass index (BMI); relationships of lifestyle factors to BMI, cardiovascular conditions, and diabetic conditions; and sex differences among autistic adults. RESULTS: Autistic adults, and particularly autistic females, exhibit unhealthy diet, exercise, and sleep patterns; they are also more likely to be underweight or obese. Limited sleep duration and high rates of sleep disturbances cannot be accounted for by epilepsy or seizure disorders. Lifestyle factors are positively related to higher risk of cardiovascular conditions among autistic males, even more than family history. LIMITATIONS: Our sample may not be representative of all autistic and non-autistic people, as it primarily comprised individuals who are white, female, have a high school education or higher, and reside in the UK. Our sampling methods may also exclude some individuals on the autism spectrum, and particularly those with moderate to severe intellectual disability. This is a cross-sectional sample that can test for relationships between factors (e.g., lifestyle factors and health outcomes) but cannot assess the direction of these relationships. CONCLUSIONS: Autistic adults are less likely to meet minimal health recommendations for diet, exercise, and sleep-and these unhealthy behaviors may relate to excess risk of cardiovascular conditions. Although the present study can only provide preliminary, correlational evidence, our findings suggest that diet, exercise, and sleep should be considered and further investigated as key targets for reducing the now widely reported and dramatically increased risks of health comorbidity and premature death among autistic individuals compared to others. Physicians should work cooperatively with patients to provide health education and develop individualized strategies for how to better manage challenges with diet, exercise, and sleep. En ligne : http://dx.doi.org/10.1186/s13229-021-00441-x Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=459 [article] An investigation of the diet, exercise, sleep, BMI, and health outcomes of autistic adults [Texte imprimé et/ou numérique] / E. WEIR, Auteur ; Carrie ALLISON, Auteur ; K. K. ONG, Auteur ; Simon BARON-COHEN, Auteur . - 31 p. Langues : Anglais (eng) in Molecular Autism > 12 (2021) . - 31 p. Mots-clés : Adult outcomes  Comorbidities  Exercise  Healthcare  Nutrition  Physical health  Sleep Index. décimale : PER Périodiques Résumé : BACKGROUND: Studies of autistic children suggest that restricted eating, reduced physical activity, and sleep disorders are common; however, no studies attempt to broadly describe the diet, exercise, and sleep patterns of autistic adults or consider relationships between lifestyle behaviors and the widely reported increased risks of obesity and chronic conditions. To address this, the authors developed the largest study of lifestyle patterns of autistic adults and assessed their relationships to body mass index, health outcomes, and family history. METHODS: We administered an anonymized, online survey to n?=?2386 adults (n?=?1183 autistic) aged 16-90 years of age. We employed Fisher's exact tests and binomial logistic regression to describe diet, exercise, and sleep patterns; mediation of seizure disorders on sleep; body mass index (BMI); relationships of lifestyle factors to BMI, cardiovascular conditions, and diabetic conditions; and sex differences among autistic adults. RESULTS: Autistic adults, and particularly autistic females, exhibit unhealthy diet, exercise, and sleep patterns; they are also more likely to be underweight or obese. Limited sleep duration and high rates of sleep disturbances cannot be accounted for by epilepsy or seizure disorders. Lifestyle factors are positively related to higher risk of cardiovascular conditions among autistic males, even more than family history. LIMITATIONS: Our sample may not be representative of all autistic and non-autistic people, as it primarily comprised individuals who are white, female, have a high school education or higher, and reside in the UK. Our sampling methods may also exclude some individuals on the autism spectrum, and particularly those with moderate to severe intellectual disability. This is a cross-sectional sample that can test for relationships between factors (e.g., lifestyle factors and health outcomes) but cannot assess the direction of these relationships. CONCLUSIONS: Autistic adults are less likely to meet minimal health recommendations for diet, exercise, and sleep-and these unhealthy behaviors may relate to excess risk of cardiovascular conditions. Although the present study can only provide preliminary, correlational evidence, our findings suggest that diet, exercise, and sleep should be considered and further investigated as key targets for reducing the now widely reported and dramatically increased risks of health comorbidity and premature death among autistic individuals compared to others. Physicians should work cooperatively with patients to provide health education and develop individualized strategies for how to better manage challenges with diet, exercise, and sleep. En ligne : http://dx.doi.org/10.1186/s13229-021-00441-x Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=459

An investigation of the 'female camouflage effect' in autism using a computerized ADOS-2 and a test of sex/gender differences / A. RYNKIEWICZ in Molecular Autism, 7 (2016)  

Public ISBD [article]  inMolecular Autism > 7 (2016) . - 10p. Titre : An investigation of the 'female camouflage effect' in autism using a computerized ADOS-2 and a test of sex/gender differences Type de document : Texte imprimé et/ou numérique Auteurs : A. RYNKIEWICZ, Auteur ; B. SCHULLER, Auteur ; E. MARCHI, Auteur ; S. PIANA, Auteur ; A. CAMURRI, Auteur ; A. LASSALLE, Auteur ; Simon BARON-COHEN, Auteur Article en page(s) : 10p. Langues : Anglais (eng) Mots-clés : Asperger Syndrome/diagnosis/psychology  Autistic Disorder/diagnosis/psychology  Child  Child, Preschool  Communication Disorders/diagnosis/etiology  Culture  Diagnosis, Computer-Assisted/methods  Diagnostic Errors  Emotions  Facial Expression  Female  Fixation, Ocular  Gestures  Humans  Male  Parents  Poland  Psychomotor Performance  Severity of Illness Index  Sex Characteristics  Sex Factors  Social Behavior  Software  Stereotyped Behavior  Surveys and Questionnaires  Ados-2  Computer application  Diagnosis  Females with autism Index. décimale : PER Périodiques Résumé : BACKGROUND: Autism spectrum conditions (autism) are diagnosed more frequently in boys than in girls. Females with autism may have been under-identified due to not only a male-biased understanding of autism but also females' camouflaging. The study describes a new technique that allows automated coding of non-verbal mode of communication (gestures) and offers the possibility of objective, evaluation of gestures, independent of human judgment. The EyesWeb software platform and the Kinect sensor during two demonstration activities of ADOS-2 (Autism Diagnostic Observation Schedule, Second Edition) were used. METHODS: The study group consisted of 33 high-functioning Polish girls and boys with formal diagnosis of autism or Asperger syndrome aged 5-10, with fluent speech, IQ average and above and their parents (girls with autism, n = 16; boys with autism, n = 17). All children were assessed during two demonstration activities of Module 3 of ADOS-2, administered in Polish, and coded using Polish codes. Children were also assessed with Polish versions of the Eyes and Faces Tests. Parents provided information on the author-reviewed Polish research translation of SCQ (Social Communication Questionnaire, Current and Lifetime) and Polish version of AQ Child (Autism Spectrum Quotient, Child). RESULTS: Girls with autism tended to use gestures more vividly as compared to boys with autism during two demonstration activities of ADOS-2. Girls with autism made significantly more mistakes than boys with autism on the Faces Test. All children with autism had high scores in AQ Child, which confirmed the presence of autistic traits in this group. The current communication skills of boys with autism reported by parents in SCQ were significantly better than those of girls with autism. However, both girls with autism and boys with autism improved in the social and communication abilities over the lifetime. The number of stereotypic behaviours in boys significantly decreased over life whereas it remained at a comparable level in girls with autism. CONCLUSIONS: High-functioning females with autism might present better on non-verbal (gestures) mode of communication than boys with autism. It may camouflage other diagnostic features. It poses risk of under-diagnosis or not receiving the appropriate diagnosis for this population. Further research is required to examine this phenomenon so appropriate gender revisions to the diagnostic assessments might be implemented. En ligne : http://dx.doi.org/10.1186/s13229-016-0073-0 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=329 [article] An investigation of the 'female camouflage effect' in autism using a computerized ADOS-2 and a test of sex/gender differences [Texte imprimé et/ou numérique] / A. RYNKIEWICZ, Auteur ; B. SCHULLER, Auteur ; E. MARCHI, Auteur ; S. PIANA, Auteur ; A. CAMURRI, Auteur ; A. LASSALLE, Auteur ; Simon BARON-COHEN, Auteur . - 10p. Langues : Anglais (eng) in Molecular Autism > 7 (2016) . - 10p. Mots-clés : Asperger Syndrome/diagnosis/psychology  Autistic Disorder/diagnosis/psychology  Child  Child, Preschool  Communication Disorders/diagnosis/etiology  Culture  Diagnosis, Computer-Assisted/methods  Diagnostic Errors  Emotions  Facial Expression  Female  Fixation, Ocular  Gestures  Humans  Male  Parents  Poland  Psychomotor Performance  Severity of Illness Index  Sex Characteristics  Sex Factors  Social Behavior  Software  Stereotyped Behavior  Surveys and Questionnaires  Ados-2  Computer application  Diagnosis  Females with autism Index. décimale : PER Périodiques Résumé : BACKGROUND: Autism spectrum conditions (autism) are diagnosed more frequently in boys than in girls. Females with autism may have been under-identified due to not only a male-biased understanding of autism but also females' camouflaging. The study describes a new technique that allows automated coding of non-verbal mode of communication (gestures) and offers the possibility of objective, evaluation of gestures, independent of human judgment. The EyesWeb software platform and the Kinect sensor during two demonstration activities of ADOS-2 (Autism Diagnostic Observation Schedule, Second Edition) were used. METHODS: The study group consisted of 33 high-functioning Polish girls and boys with formal diagnosis of autism or Asperger syndrome aged 5-10, with fluent speech, IQ average and above and their parents (girls with autism, n = 16; boys with autism, n = 17). All children were assessed during two demonstration activities of Module 3 of ADOS-2, administered in Polish, and coded using Polish codes. Children were also assessed with Polish versions of the Eyes and Faces Tests. Parents provided information on the author-reviewed Polish research translation of SCQ (Social Communication Questionnaire, Current and Lifetime) and Polish version of AQ Child (Autism Spectrum Quotient, Child). RESULTS: Girls with autism tended to use gestures more vividly as compared to boys with autism during two demonstration activities of ADOS-2. Girls with autism made significantly more mistakes than boys with autism on the Faces Test. All children with autism had high scores in AQ Child, which confirmed the presence of autistic traits in this group. The current communication skills of boys with autism reported by parents in SCQ were significantly better than those of girls with autism. However, both girls with autism and boys with autism improved in the social and communication abilities over the lifetime. The number of stereotypic behaviours in boys significantly decreased over life whereas it remained at a comparable level in girls with autism. CONCLUSIONS: High-functioning females with autism might present better on non-verbal (gestures) mode of communication than boys with autism. It may camouflage other diagnostic features. It poses risk of under-diagnosis or not receiving the appropriate diagnosis for this population. Further research is required to examine this phenomenon so appropriate gender revisions to the diagnostic assessments might be implemented. En ligne : http://dx.doi.org/10.1186/s13229-016-0073-0 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=329

Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids / Dwaipayan ADHYA in Molecular Autism, 12 (2021)  

Public ISBD [article]  inMolecular Autism > 12 (2021) . - 4p. Titre : Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids Type de document : Texte imprimé et/ou numérique Auteurs : Dwaipayan ADHYA, Auteur ; George CHENNELL, Auteur ; James A. CROWE, Auteur ; Eva P. VALENCIA-ALARCON, Auteur ; James SEYFORTH, Auteur ; Neveen A. HOSNY, Auteur ; Marina V. YASVOINA, Auteur ; Robert FORSTER, Auteur ; Simon BARON-COHEN, Auteur ; Anthony C. VERNON, Auteur ; Deepak P. SRIVASTAVA, Auteur Article en page(s) : 4p. Langues : Anglais (eng) Index. décimale : PER Périodiques Résumé : BACKGROUND: The inability to observe relevant biological processes in vivo significantly restricts human neurodevelopmental research. Advances in appropriate in vitro model systems, including patient-specific human brain organoids and human cortical spheroids (hCSs), offer a pragmatic solution to this issue. In particular, hCSs are an accessible method for generating homogenous organoids of dorsal telencephalic fate, which recapitulate key aspects of human corticogenesis, including the formation of neural rosettes-in vitro correlates of the neural tube. These neurogenic niches give rise to neural progenitors that subsequently differentiate into neurons. Studies differentiating induced pluripotent stem cells (hiPSCs) in 2D have linked atypical formation of neural rosettes with neurodevelopmental disorders such as autism spectrum conditions. Thus far, however, conventional methods of tissue preparation in this field limit the ability to image these structures in three-dimensions within intact hCS or other 3D preparations. To overcome this limitation, we have sought to optimise a methodological approach to process hCSs to maximise the utility of a novel Airy-beam light sheet microscope (ALSM) to acquire high resolution volumetric images of internal structures within hCS representative of early developmental time points. RESULTS: Conventional approaches to imaging hCS by confocal microscopy were limited in their ability to image effectively into intact spheroids. Conversely, volumetric acquisition by ALSM offered superior imaging through intact, non-clarified, in vitro tissues, in both speed and resolution when compared to conventional confocal imaging systems. Furthermore, optimised immunohistochemistry and optical clearing of hCSs afforded improved imaging at depth. This permitted visualization of the morphology of the inner lumen of neural rosettes. CONCLUSION: We present an optimized methodology that takes advantage of an ALSM system that can rapidly image intact 3D brain organoids at high resolution while retaining a large field of view. This imaging modality can be applied to both non-cleared and cleared in vitro human brain spheroids derived from hiPSCs for precise examination of their internal 3D structures. This process represents a rapid, highly efficient method to examine and quantify in 3D the formation of key structures required for the coordination of neurodevelopmental processes in both health and disease states. We posit that this approach would facilitate investigation of human neurodevelopmental processes in vitro. En ligne : http://dx.doi.org/10.1186/s13229-021-00413-1 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=442 [article] Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids [Texte imprimé et/ou numérique] / Dwaipayan ADHYA, Auteur ; George CHENNELL, Auteur ; James A. CROWE, Auteur ; Eva P. VALENCIA-ALARCON, Auteur ; James SEYFORTH, Auteur ; Neveen A. HOSNY, Auteur ; Marina V. YASVOINA, Auteur ; Robert FORSTER, Auteur ; Simon BARON-COHEN, Auteur ; Anthony C. VERNON, Auteur ; Deepak P. SRIVASTAVA, Auteur . - 4p. Langues : Anglais (eng) in Molecular Autism > 12 (2021) . - 4p. Index. décimale : PER Périodiques Résumé : BACKGROUND: The inability to observe relevant biological processes in vivo significantly restricts human neurodevelopmental research. Advances in appropriate in vitro model systems, including patient-specific human brain organoids and human cortical spheroids (hCSs), offer a pragmatic solution to this issue. In particular, hCSs are an accessible method for generating homogenous organoids of dorsal telencephalic fate, which recapitulate key aspects of human corticogenesis, including the formation of neural rosettes-in vitro correlates of the neural tube. These neurogenic niches give rise to neural progenitors that subsequently differentiate into neurons. Studies differentiating induced pluripotent stem cells (hiPSCs) in 2D have linked atypical formation of neural rosettes with neurodevelopmental disorders such as autism spectrum conditions. Thus far, however, conventional methods of tissue preparation in this field limit the ability to image these structures in three-dimensions within intact hCS or other 3D preparations. To overcome this limitation, we have sought to optimise a methodological approach to process hCSs to maximise the utility of a novel Airy-beam light sheet microscope (ALSM) to acquire high resolution volumetric images of internal structures within hCS representative of early developmental time points. RESULTS: Conventional approaches to imaging hCS by confocal microscopy were limited in their ability to image effectively into intact spheroids. Conversely, volumetric acquisition by ALSM offered superior imaging through intact, non-clarified, in vitro tissues, in both speed and resolution when compared to conventional confocal imaging systems. Furthermore, optimised immunohistochemistry and optical clearing of hCSs afforded improved imaging at depth. This permitted visualization of the morphology of the inner lumen of neural rosettes. CONCLUSION: We present an optimized methodology that takes advantage of an ALSM system that can rapidly image intact 3D brain organoids at high resolution while retaining a large field of view. This imaging modality can be applied to both non-cleared and cleared in vitro human brain spheroids derived from hiPSCs for precise examination of their internal 3D structures. This process represents a rapid, highly efficient method to examine and quantify in 3D the formation of key structures required for the coordination of neurodevelopmental processes in both health and disease states. We posit that this approach would facilitate investigation of human neurodevelopmental processes in vitro. En ligne : http://dx.doi.org/10.1186/s13229-021-00413-1 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=442

Apprendre aux enfants autistes à comprendre la pensée des autres / Patricia HOWLIN

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Apprendre aux enfants autistes à comprendre la pensée des autres / Patricia HOWLIN

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Apprendre aux enfants autistes à comprendre la pensée des autres / Patricia HOWLIN

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Apprendre aux enfants autistes à comprendre la pensée des autres / Patricia HOWLIN

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Are Autism Spectrum Conditions More Prevalent in an Information-Technology Region? A School-Based Study of Three Regions in the Netherlands / Martine T. ROELFSEMA in Journal of Autism and Developmental Disorders, 42-5 (May 2012)  

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Are autistic people disadvantaged by the criminal justice system? A case comparison / Rachel SLAVNY-CROSS in Autism, 27-5 (July 2023)  

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Asperger Syndrome / Simon BARON-COHEN

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Assessing Autism in Adults: An Evaluation of the Developmental, Dimensional and Diagnostic Interview-Adult Version (3Di-Adult) / W. MANDY in Journal of Autism and Developmental Disorders, 48-2 (February 2018)  

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Atypical integration of social cues for orienting to gaze direction in adults with autism / Chris ASHWIN in Molecular Autism, (January 2015)  

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Atypical lateralization of motor circuit functional connectivity in children with autism is associated with motor deficits / D. L. FLORIS in Molecular Autism, 7 (2016)  

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