[article]
| Titre : |
Placental methylome analysis from a prospective autism study |
| Type de document : |
Texte imprimé et/ou numérique |
| Auteurs : |
D. I. SCHROEDER, Auteur ; Rebecca J. SCHMIDT, Auteur ; F. K. CRARY-DOOLEY, Auteur ; Cheryl K. WALKER, Auteur ; S. OZONOFF, Auteur ; Daniel J. TANCREDI, Auteur ; I. HERTZ-PICCIOTTO, Auteur ; J. M. LASALLE, Auteur |
| Article en page(s) : |
51p. |
| Langues : |
Anglais (eng) |
| Mots-clés : |
Autism Spectrum Disorder/diagnosis/genetics Biomarkers/metabolism Child, Preschool DNA Methylation Early Diagnosis Enhancer Elements, Genetic Epigenesis, Genetic Female Genome, Human Genome-Wide Association Study High-Throughput Nucleotide Sequencing Humans Infant, Newborn Intercellular Signaling Peptides and Proteins/genetics/metabolism Male Membrane Proteins/genetics/metabolism Placenta/metabolism Pregnancy Biomarkers DNA methylation Epigenetics Genomics Methylome Placenta |
| Index. décimale : |
PER Périodiques |
| Résumé : |
BACKGROUND: Autism spectrum disorders (ASD) are increasingly prevalent neurodevelopmental disorders that are behaviorally diagnosed in early childhood. Most ASD cases likely arise from a complex mixture of genetic and environmental factors, an interface where the epigenetic marks of DNA methylation may be useful as risk biomarkers. The placenta is a potentially useful surrogate tissue characterized by a methylation pattern of partially methylated domains (PMDs) and highly methylated domains (HMDs) reflective of methylation patterns observed in the early embryo. METHODS: In this study, we investigated human term placentas from the MARBLES (Markers of Autism Risk in Babies: Learning Early Signs) prospective study by whole genome bisulfite sequencing. We also examined the utility of PMD/HMDs in detecting methylation differences consistent with ASD diagnosis at age three. RESULTS: We found that while human placental methylomes have highly reproducible PMD and HMD locations, there is a greater variation between individuals in methylation levels over PMDs than HMDs due to both sampling and individual variability. In a comparison of methylation differences in placental samples from 24 ASD and 23 typically developing (TD) children, a HMD containing a putative fetal brain enhancer near DLL1 was found to reach genome-wide significance and was validated for significantly higher methylation in ASD by pyrosequencing. CONCLUSIONS: These results suggest that the placenta could be an informative surrogate tissue for predictive ASD biomarkers in high-risk families. |
| En ligne : |
http://dx.doi.org/10.1186/s13229-016-0114-8 |
| Permalink : |
https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=329 |
in Molecular Autism > 7 (2016) . - 51p.
|
Placental methylome analysis from a prospective autism study in Molecular Autism, 7 (2016)
