Granulocyte macrophage colony-stimulating factor-induced macrophages of individuals with autism spectrum disorder adversely affect neuronal dendrites through the secretion of pro-inflammatory cytokines / Ryohei TAKADA in Molecular Autism, 15 (2024)  

Public ISBD [article]  inMolecular Autism > 15 (2024) . - 10p. Titre : Granulocyte macrophage colony-stimulating factor-induced macrophages of individuals with autism spectrum disorder adversely affect neuronal dendrites through the secretion of pro-inflammatory cytokines Type de document : Texte imprimé et/ou numérique Auteurs : Ryohei TAKADA, Auteur ; Michihiro TORITSUKA, Auteur ; Takahira YAMAUCHI, Auteur ; Rio ISHIDA, Auteur ; Yoshinori KAYASHIMA, Auteur ; Yuki NISHI, Auteur ; Mitsuru ISHIKAWA, Auteur ; Kazuhiko YAMAMURO, Auteur ; Minobu IKEHARA, Auteur ; Takashi KOMORI, Auteur ; Yuki NORIYAMA, Auteur ; Kohei KAMIKAWA, Auteur ; Yasuhiko SAITO, Auteur ; Hideyuki OKANO, Auteur ; Manabu MAKINODAN, Auteur Article en page(s) : 10p. Langues : Anglais (eng) Mots-clés : Female  Male  Humans  Cytokines  Granulocyte-Macrophage Colony-Stimulating Factor/metabolism/pharmacology  Macrophage Colony-Stimulating Factor/metabolism/pharmacology  Tumor Necrosis Factor-alpha/metabolism/pharmacology  Leukocytes, Mononuclear/metabolism  Interleukin-1alpha/metabolism/pharmacology  Autism Spectrum Disorder/metabolism  Cells, Cultured  Sexism  Macrophages/metabolism  Granulocytes/metabolism  Dendrites/metabolism  Autism spectrum disorder  Dendrite  Human iPS cell  Interleukin-1?  Macrophage  Tumor necrosis factor-? Index. décimale : PER Périodiques Résumé : BACKGROUND: A growing body of evidence suggests that immune dysfunction and inflammation in the peripheral tissues as well as the central nervous system are associated with the neurodevelopmental deficits observed in autism spectrum disorder (ASD). Elevated expression of pro-inflammatory cytokines in the plasma, serum, and peripheral blood mononuclear cells of ASD has been reported. These cytokine expression levels are associated with the severity of behavioral impairments and symptoms in ASD. In a prior study, our group reported that tumor necrosis factor-? (TNF-?) expression in granulocyte-macrophage colony-stimulating factor-induced macrophages (GM-CSF M?) and the TNF-? expression ratio in GM-CSF M?/M-CSF M? (macrophage colony-stimulating factor-induced macrophages) was markedly higher in individuals with ASD than in typically developed (TD) individuals. However, the mechanisms of how the macrophages and the highly expressed cytokines affect neurons remain to be addressed. METHODS: To elucidate the effect of macrophages on human neurons, we used a co-culture system of control human-induced pluripotent stem cell-derived neurons and differentiated macrophages obtained from the peripheral blood mononuclear cells of five TD individuals and five individuals with ASD. All participants were male and ethnically Japanese. RESULTS: Our results of co-culture experiments showed that GM-CSF M? affect the dendritic outgrowth of neurons through the secretion of pro-inflammatory cytokines, interleukin-1? and TNF-?. Macrophages derived from individuals with ASD exerted more severe effects than those derived from TD individuals. LIMITATIONS: The main limitations of our study were the small sample size with a gender bias toward males, the use of artificially polarized macrophages, and the inability to directly observe the interaction between neurons and macrophages from the same individuals. CONCLUSIONS: Our co-culture system revealed the non-cell autonomous adverse effects of GM-CSF M? in individuals with ASD on neurons, mediated by interleukin-1? and TNF-?. These results may support the immune dysfunction hypothesis of ASD, providing new insights into its pathology. En ligne : https://dx.doi.org/10.1186/s13229-024-00589-2 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=538 [article] Granulocyte macrophage colony-stimulating factor-induced macrophages of individuals with autism spectrum disorder adversely affect neuronal dendrites through the secretion of pro-inflammatory cytokines [Texte imprimé et/ou numérique] / Ryohei TAKADA, Auteur ; Michihiro TORITSUKA, Auteur ; Takahira YAMAUCHI, Auteur ; Rio ISHIDA, Auteur ; Yoshinori KAYASHIMA, Auteur ; Yuki NISHI, Auteur ; Mitsuru ISHIKAWA, Auteur ; Kazuhiko YAMAMURO, Auteur ; Minobu IKEHARA, Auteur ; Takashi KOMORI, Auteur ; Yuki NORIYAMA, Auteur ; Kohei KAMIKAWA, Auteur ; Yasuhiko SAITO, Auteur ; Hideyuki OKANO, Auteur ; Manabu MAKINODAN, Auteur . - 10p. Langues : Anglais (eng) in Molecular Autism > 15 (2024) . - 10p. Mots-clés : Female  Male  Humans  Cytokines  Granulocyte-Macrophage Colony-Stimulating Factor/metabolism/pharmacology  Macrophage Colony-Stimulating Factor/metabolism/pharmacology  Tumor Necrosis Factor-alpha/metabolism/pharmacology  Leukocytes, Mononuclear/metabolism  Interleukin-1alpha/metabolism/pharmacology  Autism Spectrum Disorder/metabolism  Cells, Cultured  Sexism  Macrophages/metabolism  Granulocytes/metabolism  Dendrites/metabolism  Autism spectrum disorder  Dendrite  Human iPS cell  Interleukin-1?  Macrophage  Tumor necrosis factor-? Index. décimale : PER Périodiques Résumé : BACKGROUND: A growing body of evidence suggests that immune dysfunction and inflammation in the peripheral tissues as well as the central nervous system are associated with the neurodevelopmental deficits observed in autism spectrum disorder (ASD). Elevated expression of pro-inflammatory cytokines in the plasma, serum, and peripheral blood mononuclear cells of ASD has been reported. These cytokine expression levels are associated with the severity of behavioral impairments and symptoms in ASD. In a prior study, our group reported that tumor necrosis factor-? (TNF-?) expression in granulocyte-macrophage colony-stimulating factor-induced macrophages (GM-CSF M?) and the TNF-? expression ratio in GM-CSF M?/M-CSF M? (macrophage colony-stimulating factor-induced macrophages) was markedly higher in individuals with ASD than in typically developed (TD) individuals. However, the mechanisms of how the macrophages and the highly expressed cytokines affect neurons remain to be addressed. METHODS: To elucidate the effect of macrophages on human neurons, we used a co-culture system of control human-induced pluripotent stem cell-derived neurons and differentiated macrophages obtained from the peripheral blood mononuclear cells of five TD individuals and five individuals with ASD. All participants were male and ethnically Japanese. RESULTS: Our results of co-culture experiments showed that GM-CSF M? affect the dendritic outgrowth of neurons through the secretion of pro-inflammatory cytokines, interleukin-1? and TNF-?. Macrophages derived from individuals with ASD exerted more severe effects than those derived from TD individuals. LIMITATIONS: The main limitations of our study were the small sample size with a gender bias toward males, the use of artificially polarized macrophages, and the inability to directly observe the interaction between neurons and macrophages from the same individuals. CONCLUSIONS: Our co-culture system revealed the non-cell autonomous adverse effects of GM-CSF M? in individuals with ASD on neurons, mediated by interleukin-1? and TNF-?. These results may support the immune dysfunction hypothesis of ASD, providing new insights into its pathology. En ligne : https://dx.doi.org/10.1186/s13229-024-00589-2 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=538

Microstructural Anomalies Evaluated by Neurite Orientation Dispersion and Density Imaging Are Related to Deficits in Facial Emotional Recognition via Perceptual-Binding Difficulties in Autism Spectrum Disorder / Fumihiko YASUNO in Autism Research, 13-5 (May 2020)  

Public ISBD [article]  inAutism Research > 13-5 (May 2020) . - p.729-740 Titre : Microstructural Anomalies Evaluated by Neurite Orientation Dispersion and Density Imaging Are Related to Deficits in Facial Emotional Recognition via Perceptual-Binding Difficulties in Autism Spectrum Disorder Type de document : Texte imprimé et/ou numérique Auteurs : Fumihiko YASUNO, Auteur ; Manabu MAKINODAN, Auteur ; Masato TAKAHASHI, Auteur ; Kiwamu MATSUOKA, Auteur ; Hiroaki YOSHIKAWA, Auteur ; Soichiro KITAMURA, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Toshiteru MIYASAKA, Auteur ; Kimihiko KICHIKAWA, Auteur ; Toshifumi KISHIMOTO, Auteur Article en page(s) : p.729-740 Langues : Anglais (eng) Mots-clés : Noddi  autism spectrum disorder  corpus callosum  facial emotional recognition  interhemispheric  neurite-orientation dispersion and density imaging  perceptual integration Index. décimale : PER Périodiques Résumé : The integration of visual features is important for recognizing objects as a coherent whole, a key domain of difficulty in autism spectrum disorder (ASD). We tested the hypothesis that ASD patients exhibit difficulties in facial emotional recognition via perceptual binding difficulties due to weak coherence. We assessed 18 ASD and 27 typically developing individuals for their ability to identify emotional expressions from faces in pictures moving behind a narrow vertical and horizontal slit. In this task, only a single local piece of facial information was provided at any one time through the slit. Using a voxel-based analysis of neurite-orientation dispersion and density imaging (NODDI), we examined the relationship between NODDI index values at each voxel and the behavioral performance of ASD patients in the slit-viewing paradigm. ASD patients demonstrated impaired recognition of facial emotional expression only in horizontal slit-viewing. This deficit was associated with deficits in communication ability. Voxel-based analysis revealed significant negative correlations between behavioral deficits in horizontal slit-viewing and NODDI index values in clusters including the ventral occipital complex region, superior temporal/parietal association areas, and forceps major of the corpus callosum. Our results indicated deficits for the first time in perceptual integration of facial expression across hemispheres in ASD patients due to microstructural disturbances in the corpus callosum and areas related to viewing of the human face. This may underscore the difficulties faced by ASD patients in understanding the emotions of other people, contributing to impairments in communication ability in ASD patients. Autism Res 2020, 13: 729-740. (c) 2020 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: We assessed ASD and typically developing individuals for their ability to identify emotional expressions from faces in pictures moving behind a narrow vertical and horizontal slit. ASD patients demonstrated impaired recognition of facial emotional expression only in horizontal slit-viewing. Voxel-based analysis revealed significant negative correlations between behavioral deficits and NODDI index values in clusters including the corpus callosum. Our results indicated deficits in perceptual integration of facial expression across hemispheres in ASD patients potentially resulting from microstructural disturbances. En ligne : http://dx.doi.org/10.1002/aur.2280 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=422 [article] Microstructural Anomalies Evaluated by Neurite Orientation Dispersion and Density Imaging Are Related to Deficits in Facial Emotional Recognition via Perceptual-Binding Difficulties in Autism Spectrum Disorder [Texte imprimé et/ou numérique] / Fumihiko YASUNO, Auteur ; Manabu MAKINODAN, Auteur ; Masato TAKAHASHI, Auteur ; Kiwamu MATSUOKA, Auteur ; Hiroaki YOSHIKAWA, Auteur ; Soichiro KITAMURA, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Toshiteru MIYASAKA, Auteur ; Kimihiko KICHIKAWA, Auteur ; Toshifumi KISHIMOTO, Auteur . - p.729-740. Langues : Anglais (eng) in Autism Research > 13-5 (May 2020) . - p.729-740 Mots-clés : Noddi  autism spectrum disorder  corpus callosum  facial emotional recognition  interhemispheric  neurite-orientation dispersion and density imaging  perceptual integration Index. décimale : PER Périodiques Résumé : The integration of visual features is important for recognizing objects as a coherent whole, a key domain of difficulty in autism spectrum disorder (ASD). We tested the hypothesis that ASD patients exhibit difficulties in facial emotional recognition via perceptual binding difficulties due to weak coherence. We assessed 18 ASD and 27 typically developing individuals for their ability to identify emotional expressions from faces in pictures moving behind a narrow vertical and horizontal slit. In this task, only a single local piece of facial information was provided at any one time through the slit. Using a voxel-based analysis of neurite-orientation dispersion and density imaging (NODDI), we examined the relationship between NODDI index values at each voxel and the behavioral performance of ASD patients in the slit-viewing paradigm. ASD patients demonstrated impaired recognition of facial emotional expression only in horizontal slit-viewing. This deficit was associated with deficits in communication ability. Voxel-based analysis revealed significant negative correlations between behavioral deficits in horizontal slit-viewing and NODDI index values in clusters including the ventral occipital complex region, superior temporal/parietal association areas, and forceps major of the corpus callosum. Our results indicated deficits for the first time in perceptual integration of facial expression across hemispheres in ASD patients due to microstructural disturbances in the corpus callosum and areas related to viewing of the human face. This may underscore the difficulties faced by ASD patients in understanding the emotions of other people, contributing to impairments in communication ability in ASD patients. Autism Res 2020, 13: 729-740. (c) 2020 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: We assessed ASD and typically developing individuals for their ability to identify emotional expressions from faces in pictures moving behind a narrow vertical and horizontal slit. ASD patients demonstrated impaired recognition of facial emotional expression only in horizontal slit-viewing. Voxel-based analysis revealed significant negative correlations between behavioral deficits and NODDI index values in clusters including the corpus callosum. Our results indicated deficits in perceptual integration of facial expression across hemispheres in ASD patients potentially resulting from microstructural disturbances. En ligne : http://dx.doi.org/10.1002/aur.2280 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=422

The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder / Sachie KANEKO in Autism Research, 13-6 (June 2020)  

Public ISBD [article]  inAutism Research > 13-6 (June 2020) . - p.947-958 Titre : The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder Type de document : Texte imprimé et/ou numérique Auteurs : Sachie KANEKO, Auteur ; Takahiro A. KATO, Auteur ; Manabu MAKINODAN, Auteur ; Takashi KOMORI, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Masato TAKAHASHI, Auteur ; Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidemi IWASAKA, Auteur ; Ayumi TANAKA, Auteur ; Yukiko UCHIDA, Auteur ; Shigenobu KANBA, Auteur ; Toshifumi KISHIMOTO, Auteur Article en page(s) : p.947-958 Langues : Anglais (eng) Mots-clés : attention deficit/hyperactivity disorder  autism spectrum disorder  heterogeneity  self-construal scale  well-being Index. décimale : PER Périodiques Résumé : Despite accumulating evidence that culture shapes the symptoms of autism spectrum disorder (ASD), no studies have yet applied the Self-Construal Scale to individuals with ASD. We compared the self-construals (measured using the Self-Construal Scale) of 31 high-functioning Japanese individuals with ASD with those of 60 typically developing (TD) individuals. We also examined how the self-construals of individuals with ASD related to their intelligence quotient, adverse childhood experiences, attention deficit hyperactivity disorder, ASD symptoms during adulthood and preschool years, and subjective well-being. Individuals with ASD were more likely to display independent self-construals than were TD individuals; unexpectedly, however, a substantial proportion of individuals with ASD (43.8%) displayed relatively interdependent self-construals. Among individuals with ASD, self-construals were significantly associated with ASD symptoms during preschool years, and with satisfaction of the need for autonomy and frustration of the need for relatedness. Evaluating self-construals can help predict the subjective well-being of high-functioning individuals with ASD. Moreover, the Self-Construal Scale may be useful for understanding the heterogeneous phenotypes of ASD, based on its association with autistic symptoms during preschool years, suggesting that the scale is a potential tool to develop efficient interventions for high-functioning individuals with ASD. Autism Res 2020, 13: 947-958. © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Autism Spectrum Disorders (ASD) are a group of disorders presenting a variety of symptoms and biological origins that can complicate choosing an intervention best suited for improving well-being. Results indicate that a self-construal scale could help understand individuals with high-functioning ASD by independent and interdependent self-construals that are associated with ASD symptoms during preschool years and adult subjective well-being. Our findings suggest that this scale can help understand ASD and select appropriate interventions. En ligne : http://dx.doi.org/10.1002/aur.2242 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=427 [article] The Self-Construal Scale: A Potential Tool for Predicting Subjective Well-Being of Individuals With Autism Spectrum Disorder [Texte imprimé et/ou numérique] / Sachie KANEKO, Auteur ; Takahiro A. KATO, Auteur ; Manabu MAKINODAN, Auteur ; Takashi KOMORI, Auteur ; Rio ISHIDA, Auteur ; Naoko KISHIMOTO, Auteur ; Masato TAKAHASHI, Auteur ; Yuka YASUDA, Auteur ; Ryota HASHIMOTO, Auteur ; Hidemi IWASAKA, Auteur ; Ayumi TANAKA, Auteur ; Yukiko UCHIDA, Auteur ; Shigenobu KANBA, Auteur ; Toshifumi KISHIMOTO, Auteur . - p.947-958. Langues : Anglais (eng) in Autism Research > 13-6 (June 2020) . - p.947-958 Mots-clés : attention deficit/hyperactivity disorder  autism spectrum disorder  heterogeneity  self-construal scale  well-being Index. décimale : PER Périodiques Résumé : Despite accumulating evidence that culture shapes the symptoms of autism spectrum disorder (ASD), no studies have yet applied the Self-Construal Scale to individuals with ASD. We compared the self-construals (measured using the Self-Construal Scale) of 31 high-functioning Japanese individuals with ASD with those of 60 typically developing (TD) individuals. We also examined how the self-construals of individuals with ASD related to their intelligence quotient, adverse childhood experiences, attention deficit hyperactivity disorder, ASD symptoms during adulthood and preschool years, and subjective well-being. Individuals with ASD were more likely to display independent self-construals than were TD individuals; unexpectedly, however, a substantial proportion of individuals with ASD (43.8%) displayed relatively interdependent self-construals. Among individuals with ASD, self-construals were significantly associated with ASD symptoms during preschool years, and with satisfaction of the need for autonomy and frustration of the need for relatedness. Evaluating self-construals can help predict the subjective well-being of high-functioning individuals with ASD. Moreover, the Self-Construal Scale may be useful for understanding the heterogeneous phenotypes of ASD, based on its association with autistic symptoms during preschool years, suggesting that the scale is a potential tool to develop efficient interventions for high-functioning individuals with ASD. Autism Res 2020, 13: 947-958. © 2019 International Society for Autism Research, Wiley Periodicals, Inc. LAY SUMMARY: Autism Spectrum Disorders (ASD) are a group of disorders presenting a variety of symptoms and biological origins that can complicate choosing an intervention best suited for improving well-being. Results indicate that a self-construal scale could help understand individuals with high-functioning ASD by independent and interdependent self-construals that are associated with ASD symptoms during preschool years and adult subjective well-being. Our findings suggest that this scale can help understand ASD and select appropriate interventions. En ligne : http://dx.doi.org/10.1002/aur.2242 Permalink : https://www.cra-rhone-alpes.org/cid/opac_css/index.php?lvl=notice_display&id=427

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